Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.
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