Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

Kai Tang, Dongjing Fu, Sven Kötter, Robert J. Cotter, Charles R. Cantor, Hubert Köster

Research output: Contribution to journalArticlepeer-review

Abstract

Matrix-assisted laser desorption/ionizatlon (MALDI) tlme-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidln-coated magnetic beads or controlled pore glass beads). Only the non-Immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxyplcolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

Original languageEnglish (US)
Pages (from-to)3126-3131
Number of pages6
JournalNucleic acids research
Volume23
Issue number16
DOIs
StatePublished - Aug 25 1995

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes'. Together they form a unique fingerprint.

Cite this