Maternal inflammation leads to different mTORC1 activity varied by anatomic locations in mouse placenta

Jie Dong, Na Shin, Ji Yeon Lee, Bei Jia, Anna Chudnovets, Michael W. Mclane, Su Li, Quan Na, Jun Lei, Irina Burd

Research output: Contribution to journalArticle

Abstract

Maternal inflammation (MI) is associated with many adverse perinatal outcomes. The placenta plays a vital role in mediating maternal-fetal resource allocation. Studies have shown that MI contributes to placental dysfunction, which then leads to adverse birth outcomes and high health risks throughout childhood. Placental mammalian target of rapamycin complex 1 (mTORC1) signaling pathway links maternal nutrient availability to fetal growth; however, the impact of MI on mTORC1 signaling in the placenta remains unclear. In this study, we sought to explore the changes of mTORC1 signaling in the mouse placenta at late gestation by using two models of MI employing lipopolysaccharide (LPS) and interleukin-1β (IL-1β) to mimic acute (aMI) and sub-chronic (cMI) inflammatory states, respectively. We determined placental mTORC1 activity by measuring the activity of mTORC1 downstream molecules, including S6k, 4Ebp1, and rpS6. In the aMI model, we found that mTORC1 activity was significantly decreased in the placental decidual and junctional zone at 2 and 6 h after LPS surgery, respectively; however, mTORC1 activity was significantly increased in the placental labyrinth zone at 2, 6, and 24 h after LPS treatment, respectively. In the cMI model, we observed that mTORC1 activity was increased only in the placental labyrinth zone after consecutive IL-1β exposure. Our study reveals that different parts of the mouse placenta react differently to MI, leading to variable mTORC1 activity throughout the placenta. This suggests that different downstream molecules of mTORC1 from different parts of the mouse placenta may be used in clinical research to monitor the fetal well-being during MI.

Original languageEnglish (US)
Pages (from-to)1046-1055
Number of pages10
JournalBiology of Reproduction
Volume101
Issue number5
DOIs
StatePublished - Nov 21 2019

Fingerprint

Placenta
Mothers
Inflammation
Lipopolysaccharides
Inner Ear
Interleukin-1
mechanistic target of rapamycin complex 1
Resource Allocation
Fetal Development
Parturition
Food
Pregnancy
Health
Research

Keywords

  • fetal development
  • maternal inflammation
  • nutrition
  • placenta
  • pregnancy
  • stress
  • trophoblast

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Dong, J., Shin, N., Lee, J. Y., Jia, B., Chudnovets, A., Mclane, M. W., ... Burd, I. (2019). Maternal inflammation leads to different mTORC1 activity varied by anatomic locations in mouse placenta. Biology of Reproduction, 101(5), 1046-1055. https://doi.org/10.1093/biolre/ioz151

Maternal inflammation leads to different mTORC1 activity varied by anatomic locations in mouse placenta. / Dong, Jie; Shin, Na; Lee, Ji Yeon; Jia, Bei; Chudnovets, Anna; Mclane, Michael W.; Li, Su; Na, Quan; Lei, Jun; Burd, Irina.

In: Biology of Reproduction, Vol. 101, No. 5, 21.11.2019, p. 1046-1055.

Research output: Contribution to journalArticle

Dong, J, Shin, N, Lee, JY, Jia, B, Chudnovets, A, Mclane, MW, Li, S, Na, Q, Lei, J & Burd, I 2019, 'Maternal inflammation leads to different mTORC1 activity varied by anatomic locations in mouse placenta', Biology of Reproduction, vol. 101, no. 5, pp. 1046-1055. https://doi.org/10.1093/biolre/ioz151
Dong, Jie ; Shin, Na ; Lee, Ji Yeon ; Jia, Bei ; Chudnovets, Anna ; Mclane, Michael W. ; Li, Su ; Na, Quan ; Lei, Jun ; Burd, Irina. / Maternal inflammation leads to different mTORC1 activity varied by anatomic locations in mouse placenta. In: Biology of Reproduction. 2019 ; Vol. 101, No. 5. pp. 1046-1055.
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