TY - JOUR
T1 - Maternal immunostimulation of a teratocarcinoma-derived cell line, TerCs
AU - Fenderson, Bruce A.
AU - Bartlett, Perry F.
AU - Edidin, Michael
N1 - Funding Information:
The authors wish to thank Dr. Marilyn S. Hamilton for her critical review of the manuscript. This work was supported by a grant AI 14584 to M.E. and a training grant to the Department of Biology. It is part of the Ph.D. thesis of B.F. and is contribution No. 1202 from the Department of Biology.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1983/9
Y1 - 1983/9
N2 - Since murine teratocarcinomas and early embryos are known to share cell surface antigens, we investigated the possibility of maternal immune responses to normal pregnancy using teratocarcinoma-derived cell lines as targets. We found that an adherent cell population from both the spleen and peritoneum of syngeneically mated 129/SvS1 pregnant females stimulated the uptake of [125I]iododeoxyuridine ([125I]IUdR) by a teratocarcinoma-derived cell line, TerCs in vitro. Adherent cells from multiparous females did not stimulate the growth of other tumor cell lines. However, levels of natural anti-tumor activity detected in peritoneal cell populations of 129/SvS1 virgin females were greatly reduced during pregnancy. Peritoneal cells from multiparous females with growth-stimulating activity were retained on nylon-wool columns and not eliminated by treatment with anti-theta antiserum and complement. Peritoneal cells from virgin females, treated with anti-theta antiserum and complement to eliminate cytotoxic lymphocytes, gained the ability to stimulate the uptake of [125I]IUdR by TerCs cells. [125I]IUdR uptake by cultured normal mouse blastocysts was significantly enhanced by peritoneal cells from multiparous females, while cells from age-matched virgin females had no effect. These results suggest that changes in immunocyte populations occur during pregnancy in the mouse; these changes could promote the growth of the embryo in utero.
AB - Since murine teratocarcinomas and early embryos are known to share cell surface antigens, we investigated the possibility of maternal immune responses to normal pregnancy using teratocarcinoma-derived cell lines as targets. We found that an adherent cell population from both the spleen and peritoneum of syngeneically mated 129/SvS1 pregnant females stimulated the uptake of [125I]iododeoxyuridine ([125I]IUdR) by a teratocarcinoma-derived cell line, TerCs in vitro. Adherent cells from multiparous females did not stimulate the growth of other tumor cell lines. However, levels of natural anti-tumor activity detected in peritoneal cell populations of 129/SvS1 virgin females were greatly reduced during pregnancy. Peritoneal cells from multiparous females with growth-stimulating activity were retained on nylon-wool columns and not eliminated by treatment with anti-theta antiserum and complement. Peritoneal cells from virgin females, treated with anti-theta antiserum and complement to eliminate cytotoxic lymphocytes, gained the ability to stimulate the uptake of [125I]IUdR by TerCs cells. [125I]IUdR uptake by cultured normal mouse blastocysts was significantly enhanced by peritoneal cells from multiparous females, while cells from age-matched virgin females had no effect. These results suggest that changes in immunocyte populations occur during pregnancy in the mouse; these changes could promote the growth of the embryo in utero.
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U2 - 10.1016/0165-0378(83)90255-3
DO - 10.1016/0165-0378(83)90255-3
M3 - Article
C2 - 6631836
AN - SCOPUS:0020637521
SN - 0165-0378
VL - 5
SP - 287
EP - 297
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
IS - 5
ER -