Matefin, a Caenorhabditis elegans germ line-specific SUN-domain nuclear membrane protein, is essential for early embryonic and germ cell development

Alexandra Fridkin, Erez Mills, Ayelet Margalit, Esther Neufeld, Kenneth K. Lee, Naomi Feinstein, Merav Cohen, Katherine Lee Wilson, Yosef Gruenbaum

Research output: Contribution to journalArticle

Abstract

Caenorhabditis elegans mtf-1 encodes matefin, which has a predicted SUN domain, a coiled-coil region, an anti-erbB-2 IgG domain, and two hydrophobic regions. We show that matefin is a nuclear membrane protein that colocalizes in vivo with Ce-lamin, the single nuclear lamin protein in C. elegans, and binds Ce-lamin in vitro but does not require Ce-lamin for its localization. Matefin is detected in all embryonic cells until midembryogenesis and thereafter only in germ-line cells. Embryonic matefin is maternally deposited, and matef in is the first nuclear membrane protein known to have germ line-restricted expression. Animals homozygous for an mtf-1 deletion allele show that matefin is essential for germ line maturation and survival. However, matefin is also required for embryogenesis because mtf-1 (RNAi) embryos die around the ≈300-cell stage with defects in nuclear structure, DNA content, and chromatin morphology. Down-regulating matefin in mes-3 animals only slightly enhances embryonic lethality, and elimination of UNC-84, the only other SUN-domain gene in C. elegans, has no affect on mtf-1 (RNAi) animals. Thus, mtf-1 mediates a previously uncharacterized pathway(s) required for embryogenesis as well as germ line proliferation or survival.

Original languageEnglish (US)
Pages (from-to)6987-6992
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number18
DOIs
StatePublished - May 4 2004

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Lamins
Nuclear Envelope
Caenorhabditis elegans
Nuclear Proteins
Germ Cells
Membrane Proteins
RNA Interference
Embryonic Development
Protein C
Chromatin
Embryonic Structures
Immunoglobulin G
Alleles
Embryonic Germ Cells
DNA
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Matefin, a Caenorhabditis elegans germ line-specific SUN-domain nuclear membrane protein, is essential for early embryonic and germ cell development. / Fridkin, Alexandra; Mills, Erez; Margalit, Ayelet; Neufeld, Esther; Lee, Kenneth K.; Feinstein, Naomi; Cohen, Merav; Wilson, Katherine Lee; Gruenbaum, Yosef.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 18, 04.05.2004, p. 6987-6992.

Research output: Contribution to journalArticle

Fridkin, Alexandra ; Mills, Erez ; Margalit, Ayelet ; Neufeld, Esther ; Lee, Kenneth K. ; Feinstein, Naomi ; Cohen, Merav ; Wilson, Katherine Lee ; Gruenbaum, Yosef. / Matefin, a Caenorhabditis elegans germ line-specific SUN-domain nuclear membrane protein, is essential for early embryonic and germ cell development. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 18. pp. 6987-6992.
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