Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production

A. P B Dackiw, A. B. Nathens, M. B. Ribeiro, P. Y C Cheung, J. C. Marshall, O. D. Rotstein

Research output: Contribution to journalArticle

Abstract

The expression of surface procoagulants by macrophages represents an important mechanism underlying local fibrin deposition at sites of extravascular inflammation. Mast cells by virtue of their perivascular location are in a potent position to influence the inflammatory process. The present studies investigated the role of the mast cell in the generation of macrophage procoagulant activity (PCA) and tumor necrosis factor (TNF) production. Mast cell lysates caused a marked induction of macrophage PCA (dose and time dependent) and TNF release while whole mast cells had little effect. This effect was prevented by the tyrosine kinase inhibitor herbimycin. At the molecular level, Northern blot analysis revealed marked induction of the murine macrophage tissue factor transcript in response to incubation with mast cell lysate compared to control. These studies thus suggest that mast cell-macrophage interactions promote macrophage-mediated fibrin deposition and TNF release and that this effect is in part mediated via induction of tyrosine phosphorylation. These observations suggest novel mechanisms of involvement of the mast cell in the inflammatory microenvironment and macrophage activation.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Surgical Research
Volume59
Issue number1
DOIs
StatePublished - Jul 1995
Externally publishedYes

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Mast Cells
Tumor Necrosis Factor-alpha
Macrophages
Fibrin
Macrophage Activation
Thromboplastin
macrophage procoagulant activity
Cell Communication
Northern Blotting
Protein-Tyrosine Kinases
Tyrosine
Phosphorylation
Inflammation

ASJC Scopus subject areas

  • Surgery

Cite this

Dackiw, A. P. B., Nathens, A. B., Ribeiro, M. B., Cheung, P. Y. C., Marshall, J. C., & Rotstein, O. D. (1995). Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production. Journal of Surgical Research, 59(1), 1-5. https://doi.org/10.1006/jsre.1995.1123

Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production. / Dackiw, A. P B; Nathens, A. B.; Ribeiro, M. B.; Cheung, P. Y C; Marshall, J. C.; Rotstein, O. D.

In: Journal of Surgical Research, Vol. 59, No. 1, 07.1995, p. 1-5.

Research output: Contribution to journalArticle

Dackiw, APB, Nathens, AB, Ribeiro, MB, Cheung, PYC, Marshall, JC & Rotstein, OD 1995, 'Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production', Journal of Surgical Research, vol. 59, no. 1, pp. 1-5. https://doi.org/10.1006/jsre.1995.1123
Dackiw APB, Nathens AB, Ribeiro MB, Cheung PYC, Marshall JC, Rotstein OD. Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production. Journal of Surgical Research. 1995 Jul;59(1):1-5. https://doi.org/10.1006/jsre.1995.1123
Dackiw, A. P B ; Nathens, A. B. ; Ribeiro, M. B. ; Cheung, P. Y C ; Marshall, J. C. ; Rotstein, O. D. / Mast Cell Modulation of Macrophage Procoagulant Activity and TNF Production. In: Journal of Surgical Research. 1995 ; Vol. 59, No. 1. pp. 1-5.
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