Massively parallel signature sequencing profiling of fetal human neural precursor cells

Jingli Cai, Soojung Shin, Lynda Wright, Ying Liu, Daixing Zhou, Haipeng Xue, Irina Khrebtukova, Mark P. Mattson, Clive N. Svendsen, Mahendra S. Rao

Research output: Contribution to journalArticle

Abstract

We have examined gene expression in multipotent neural precursor cells (NPCs) derived from human fetal (f) brain tissue and compared its expression profiles with embryonic stem (ESC) cells, embryoid body cell (EBC), and astrocyte precursors using the technique of massively parallel signature sequencing (MPSS). Gene expression profiles show that fNPCs express core neural stem cells markers and share expression profiles with astrocyte precursor cells (APCs) rather than ESC or EBC. Gene expression analysis shows that fNPCs differ from other adult stem and progenitor cells in their marker expression and activation of specific functional networks such as the transforming growth factorβ (TGFβ) and Notch signaling pathways. In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs.

Original languageEnglish (US)
Pages (from-to)232-244
Number of pages13
JournalStem Cells and Development
Volume15
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

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High-Throughput Nucleotide Sequencing
Embryoid Bodies
Astrocytes
Gene Expression
Adult Stem Cells
Neural Stem Cells
Transforming Growth Factors
Embryonic Stem Cells
Transcriptome
Stem Cells
Brain
Genes

ASJC Scopus subject areas

  • Hematology

Cite this

Cai, J., Shin, S., Wright, L., Liu, Y., Zhou, D., Xue, H., ... Rao, M. S. (2006). Massively parallel signature sequencing profiling of fetal human neural precursor cells. Stem Cells and Development, 15(2), 232-244. https://doi.org/10.1089/scd.2006.15.232

Massively parallel signature sequencing profiling of fetal human neural precursor cells. / Cai, Jingli; Shin, Soojung; Wright, Lynda; Liu, Ying; Zhou, Daixing; Xue, Haipeng; Khrebtukova, Irina; Mattson, Mark P.; Svendsen, Clive N.; Rao, Mahendra S.

In: Stem Cells and Development, Vol. 15, No. 2, 04.2006, p. 232-244.

Research output: Contribution to journalArticle

Cai, J, Shin, S, Wright, L, Liu, Y, Zhou, D, Xue, H, Khrebtukova, I, Mattson, MP, Svendsen, CN & Rao, MS 2006, 'Massively parallel signature sequencing profiling of fetal human neural precursor cells', Stem Cells and Development, vol. 15, no. 2, pp. 232-244. https://doi.org/10.1089/scd.2006.15.232
Cai, Jingli ; Shin, Soojung ; Wright, Lynda ; Liu, Ying ; Zhou, Daixing ; Xue, Haipeng ; Khrebtukova, Irina ; Mattson, Mark P. ; Svendsen, Clive N. ; Rao, Mahendra S. / Massively parallel signature sequencing profiling of fetal human neural precursor cells. In: Stem Cells and Development. 2006 ; Vol. 15, No. 2. pp. 232-244.
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