Mass spectrometry with soft ionization techniques for structural analysis of lipids in Serratia species

Hisako Hara-Hotta, Yoshiko Miyazaki, Ikuya Yano, Tohey Matsuyama, Robert J. Cotter

Research output: Contribution to journalArticlepeer-review

Abstract

Mass spectrometry with soft ionization techniques (fast atom bombardment, plasma desorption and laser desorption) was applied for structural analysis of the membrane lipids from Serratia marcescens, an opportunistic pathogen. The molecular weights of the individual phospho- and amino-lipids were determined from the quasimolecular ions. Serratamolide, the most characteristic cyclic depsipeptide in this species, was shown to contain two molecules of serine and two molecules of 3-hydroxydecanoic acid, based on the mass numbers of [M + H]+, [M + Na]+ or [M + K]+ ions in the positive mode. An unidentified cyclic depsipeptide was found; its molecular ion was 32 u higher than that of serratamolide. An unusual ornithine-containing lipid was also found; in this lipid, the carboxyl group of a 3-hydroxy fatty acid was linked to the α-amino group of ornithine and another non-polar fatty acid was linked to the hydroxyl group of the 3-hydroxy fatty acid. The results obtained with the soft ionization techniques agreed with each other and did not contradict the conventional structural analysis. It is concluded that the soft ionization techniques gave precise molecular weights for such types of lipid without any degradation.

Original languageEnglish (US)
Pages (from-to)283-293
Number of pages11
JournalAnalytica Chimica Acta
Volume247
Issue number2
DOIs
StatePublished - Jul 1 1991

Keywords

  • Lipids
  • Mass spectrometry
  • Soft ionization techniques

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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