Mass spectrometrical analysis of galectin proteins in primary rat cerebellar astrocytes

J. W. Yang, S. U. Kang, E. Engidawork, R. Rodrigo, V. Felipo, G. Lubec

Research output: Contribution to journalArticlepeer-review

Abstract

Galectins are a family of animal lectins with specificity for β-galactosides and are involved in a host of cellular activities, ranging from development to cancer. The molecules are expressed by neural and non-neural cells intracellularly as well as extracellularly. Using two-dimensional gel electrophoresis coupled to tandem mass spectrometry, the present work aimed to identify and characterize galectins in primary rat cerebellar astrocytes. The protein-chemical method identified nine spots representing two members of the galectin family, namely galectin-1 and galectin-3. These findings suggest that high abundant expression of galectin in astrocytes is limited to the two abundant galectin family members. As these family members are linked to human astrocytic tumors, their reliable detection in astrocytes by proteomic techniques would enable us to further understand their role in neural development, injury, and regeneration in general and astrocytoma in particular.

Original languageEnglish (US)
Pages (from-to)945-955
Number of pages11
JournalNeurochemical Research
Volume31
Issue number7
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

Keywords

  • Astrocytes
  • Galectin
  • Mass spectrometry
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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