TY - JOUR
T1 - Mass fractionation of Drosophila egg chambers
AU - Jacobs-Lorena, Marcelo
AU - Crippa, Marco
N1 - Funding Information:
We thank Dr. Leonard Nelson for his help in the preparation of the manuscript. This investigation was supported by Grant No. 3.318.74 from the Swiss National Fund.
PY - 1977/6
Y1 - 1977/6
N2 - Egg chambers from Drosophila ovaries may now be fractionated in large quantities suitable for biochemical work. A suspension of isolated egg chambers is prepared by subjecting ovaries to digestion with 0.067% collagenase. Egg chambers are separated in size classes (which correspond to different developmental stages) by sieving them through a column of superimposed nylon nets. The method can, in principle, be used for the separation of any particles ranging upward from 10 μm in diameter.
AB - Egg chambers from Drosophila ovaries may now be fractionated in large quantities suitable for biochemical work. A suspension of isolated egg chambers is prepared by subjecting ovaries to digestion with 0.067% collagenase. Egg chambers are separated in size classes (which correspond to different developmental stages) by sieving them through a column of superimposed nylon nets. The method can, in principle, be used for the separation of any particles ranging upward from 10 μm in diameter.
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U2 - 10.1016/0012-1606(77)90223-8
DO - 10.1016/0012-1606(77)90223-8
M3 - Article
C2 - 194805
AN - SCOPUS:0017397880
SN - 0012-1606
VL - 57
SP - 385
EP - 392
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -