Mass fractionation of Drosophila egg chambers

Marcelo Jacobs-Lorena, Marco Crippa

Research output: Contribution to journalArticle

Abstract

Egg chambers from Drosophila ovaries may now be fractionated in large quantities suitable for biochemical work. A suspension of isolated egg chambers is prepared by subjecting ovaries to digestion with 0.067% collagenase. Egg chambers are separated in size classes (which correspond to different developmental stages) by sieving them through a column of superimposed nylon nets. The method can, in principle, be used for the separation of any particles ranging upward from 10 μm in diameter.

Original languageEnglish (US)
Pages (from-to)385-392
Number of pages8
JournalDevelopmental biology
Volume57
Issue number2
DOIs
StatePublished - Jun 1977
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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