Mass correlated acceleration in a reflectron MALDI TOF mass spectrometer: An approach for enhanced resolution over a broad mass range

Slava V. Kovtoun, Robert D. English, Robert J. Cotter

Research output: Contribution to journalArticle

Abstract

Compared to continuous extraction, pulsed extraction (PE) of ions formed by matrix-assisted laser desorption/ionization (MALDI) in time-of-flight (TOF) mass spectrometers significantly improves mass resolution. Parameters such as extraction voltage, delay time, and correction pulse must be varied, however, to achieve optimum mass resolution over a broad mass range because the PE method is mass dependent. We previously reported a novel method, mass correlated acceleration (MCA), which we have now combined with a reflectron MALDI TOF mass spectrometer to further enhance mass resolution over a broader mass range. Unlike the PE method, MCA is not mass dependent and high resolution mass spectra can be achieved with a single tuning of instrument parameters. The ions may be brought into focus simultaneously, i.e., the multi-channel recording advantage can be more fully realized. The MCA dual-stage ion source design includes an extraction pulse region and an acceleration region that contains a time-dependent waveform correlated with mass. We demonstrate the validity of this novel technique with applications in peptide mixture analysis and protein digests of lysozyme and bovine serum albumin.

Original languageEnglish (US)
Pages (from-to)135-143
Number of pages9
JournalJournal of the American Society for Mass Spectrometry
Volume13
Issue number2
DOIs
StatePublished - Jun 4 2002

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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