Masking of the CBF1/RBPJκ transcriptional repression domain by Epstein-Barr virus EBNA2

James J.D. Hsieh; S. Diane Hayward

doi:10.1126/science.7725102

Masking of the CBF1/RBPJ_κ transcriptional repression domain by Epstein-Barr virus EBNA2

James J.D. Hsieh, S. Diane Hayward

School of Medicine

Research output: Contribution to journal › Article › peer-review

250 Scopus citations

Abstract

Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) is a transcriptional activator that is essential for EBV-driven B cell immortalization. EBNA2 is targeted to responsive promoters through interaction with a cellular DNA binding protein, C promoter binding factor 1 (CBF1). A transcriptional repression domain has been identified within CBF1. This domain also interacts with EBNA2, and repression is masked by EBNA2 binding. Thus, EBNA2 acts by countering transcriptional repression. Mutation at amino acid 233 of CBF1 abolishes repression and correlates with a loss-of-function mutation in the Drosophila homolog Su(H).

Original language	English (US)
Pages (from-to)	560-563
Number of pages	4
Journal	Science
Volume	268
Issue number	5210
DOIs	https://doi.org/10.1126/science.7725102
State	Published - 1995

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title = "Masking of the CBF1/RBPJκ transcriptional repression domain by Epstein-Barr virus EBNA2",

abstract = "Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) is a transcriptional activator that is essential for EBV-driven B cell immortalization. EBNA2 is targeted to responsive promoters through interaction with a cellular DNA binding protein, C promoter binding factor 1 (CBF1). A transcriptional repression domain has been identified within CBF1. This domain also interacts with EBNA2, and repression is masked by EBNA2 binding. Thus, EBNA2 acts by countering transcriptional repression. Mutation at amino acid 233 of CBF1 abolishes repression and correlates with a loss-of-function mutation in the Drosophila homolog Su(H).",

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AB - Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) is a transcriptional activator that is essential for EBV-driven B cell immortalization. EBNA2 is targeted to responsive promoters through interaction with a cellular DNA binding protein, C promoter binding factor 1 (CBF1). A transcriptional repression domain has been identified within CBF1. This domain also interacts with EBNA2, and repression is masked by EBNA2 binding. Thus, EBNA2 acts by countering transcriptional repression. Mutation at amino acid 233 of CBF1 abolishes repression and correlates with a loss-of-function mutation in the Drosophila homolog Su(H).

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Masking of the CBF1/RBPJ_κ transcriptional repression domain by Epstein-Barr virus EBNA2

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