Masked hypodiploidy

Hypodiploid acute lymphoblastic leukemia (ALL) mimicking hyperdiploid ALL in children: A report from the Children's Oncology Group

Andrew J. Carroll, Mary Shago, Fady M. Mikhail, Susana C. Raimondi, Betsy A. Hirsch, Mignon L. Loh, Elizabeth A. Raetz, Michael J Borowitz, Brent L. Wood, Kelly W. Maloney, Leonard A. Mattano, Eric C. Larsen, J. Gastier-Foster, Eileen Stonerock, Denise Ell, S. Kahwash, Meenakshi Devidas, Richard C. Harvey, I. Ming L. Chen, Cheryl L. Willman & 5 others Stephen P. Hunger, Naomi J. Winick, William L. Carroll, Kathleen W. Rao, Nyla A. Heerema

Research output: Contribution to journalArticle

Abstract

Hyperdiploidy with greater than 50 chromosomes is usually associated with favorable prognosis in pediatric acute lymphoblastic leukemia (ALL), whereas hypodiploidy with ≤43 chromosomes is associated with extremely poor prognosis. Sometimes, hypodiploidy is “masked” and patients do not have a karyotypically visible clone with ≤43 chromosomes. Instead, their abnormal karyotypes contain 50–78 or more chromosomes from doubling of previously hypodiploid cells. When the hypodiploid and doubled hyperdiploid clones are both present, patients can be identified by traditional test methods [karyotype, DNA Index (DI), fluorescence in situ hybridization (FISH)], but the incidence of masked hypodiploid cases in which only the doubled clone is visible is unknown. We analyzed 7013 patients with B-ALL enrolled in COG AALL03B1 (2003–2011) for whom chromosome studies were available. Of 115 patients with hypodiploidy (25–39 chromosomes), karyotypes of 40 showed only the hypodiploid clone, 47 showed mosaicism with both hypodiploid and hyperdiploid (doubled) karyotypes, and 28 with masked hypodiploidy showed only a hyperdiploid (doubled) clone. Unique karyotypic signatures were identified, and widespread loss of heterozygosity (LOH) was seen in the microsatellite panel for all patients with masked hypodiploidy. An increased awareness of the unusual karyotypic profile associated with a doubled hypodiploid clone and coordinated use of DI, FISH, and LOH studies when indicated can identify patients with masked hypodiploidy and allow appropriate treatment selection.

Original languageEnglish (US)
Pages (from-to)62-68
Number of pages7
JournalCancer Genetics
Volume238
DOIs
StatePublished - Oct 1 2019

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Polyploidy
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Clone Cells
Chromosomes
Karyotype
Loss of Heterozygosity
Fluorescence In Situ Hybridization
Abnormal Karyotype
Mosaicism
DNA
Microsatellite Repeats
Pediatrics
Incidence

Keywords

  • B-ALL
  • Cytogenetics
  • Doubling
  • Hypodiploid
  • Low-hypodiploid
  • Near-haploid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Masked hypodiploidy : Hypodiploid acute lymphoblastic leukemia (ALL) mimicking hyperdiploid ALL in children: A report from the Children's Oncology Group. / Carroll, Andrew J.; Shago, Mary; Mikhail, Fady M.; Raimondi, Susana C.; Hirsch, Betsy A.; Loh, Mignon L.; Raetz, Elizabeth A.; Borowitz, Michael J; Wood, Brent L.; Maloney, Kelly W.; Mattano, Leonard A.; Larsen, Eric C.; Gastier-Foster, J.; Stonerock, Eileen; Ell, Denise; Kahwash, S.; Devidas, Meenakshi; Harvey, Richard C.; Chen, I. Ming L.; Willman, Cheryl L.; Hunger, Stephen P.; Winick, Naomi J.; Carroll, William L.; Rao, Kathleen W.; Heerema, Nyla A.

In: Cancer Genetics, Vol. 238, 01.10.2019, p. 62-68.

Research output: Contribution to journalArticle

Carroll, AJ, Shago, M, Mikhail, FM, Raimondi, SC, Hirsch, BA, Loh, ML, Raetz, EA, Borowitz, MJ, Wood, BL, Maloney, KW, Mattano, LA, Larsen, EC, Gastier-Foster, J, Stonerock, E, Ell, D, Kahwash, S, Devidas, M, Harvey, RC, Chen, IML, Willman, CL, Hunger, SP, Winick, NJ, Carroll, WL, Rao, KW & Heerema, NA 2019, 'Masked hypodiploidy: Hypodiploid acute lymphoblastic leukemia (ALL) mimicking hyperdiploid ALL in children: A report from the Children's Oncology Group', Cancer Genetics, vol. 238, pp. 62-68. https://doi.org/10.1016/j.cancergen.2019.07.009
Carroll, Andrew J. ; Shago, Mary ; Mikhail, Fady M. ; Raimondi, Susana C. ; Hirsch, Betsy A. ; Loh, Mignon L. ; Raetz, Elizabeth A. ; Borowitz, Michael J ; Wood, Brent L. ; Maloney, Kelly W. ; Mattano, Leonard A. ; Larsen, Eric C. ; Gastier-Foster, J. ; Stonerock, Eileen ; Ell, Denise ; Kahwash, S. ; Devidas, Meenakshi ; Harvey, Richard C. ; Chen, I. Ming L. ; Willman, Cheryl L. ; Hunger, Stephen P. ; Winick, Naomi J. ; Carroll, William L. ; Rao, Kathleen W. ; Heerema, Nyla A. / Masked hypodiploidy : Hypodiploid acute lymphoblastic leukemia (ALL) mimicking hyperdiploid ALL in children: A report from the Children's Oncology Group. In: Cancer Genetics. 2019 ; Vol. 238. pp. 62-68.
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T2 - Hypodiploid acute lymphoblastic leukemia (ALL) mimicking hyperdiploid ALL in children: A report from the Children's Oncology Group

AU - Carroll, Andrew J.

AU - Shago, Mary

AU - Mikhail, Fady M.

AU - Raimondi, Susana C.

AU - Hirsch, Betsy A.

AU - Loh, Mignon L.

AU - Raetz, Elizabeth A.

AU - Borowitz, Michael J

AU - Wood, Brent L.

AU - Maloney, Kelly W.

AU - Mattano, Leonard A.

AU - Larsen, Eric C.

AU - Gastier-Foster, J.

AU - Stonerock, Eileen

AU - Ell, Denise

AU - Kahwash, S.

AU - Devidas, Meenakshi

AU - Harvey, Richard C.

AU - Chen, I. Ming L.

AU - Willman, Cheryl L.

AU - Hunger, Stephen P.

AU - Winick, Naomi J.

AU - Carroll, William L.

AU - Rao, Kathleen W.

AU - Heerema, Nyla A.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Hyperdiploidy with greater than 50 chromosomes is usually associated with favorable prognosis in pediatric acute lymphoblastic leukemia (ALL), whereas hypodiploidy with ≤43 chromosomes is associated with extremely poor prognosis. Sometimes, hypodiploidy is “masked” and patients do not have a karyotypically visible clone with ≤43 chromosomes. Instead, their abnormal karyotypes contain 50–78 or more chromosomes from doubling of previously hypodiploid cells. When the hypodiploid and doubled hyperdiploid clones are both present, patients can be identified by traditional test methods [karyotype, DNA Index (DI), fluorescence in situ hybridization (FISH)], but the incidence of masked hypodiploid cases in which only the doubled clone is visible is unknown. We analyzed 7013 patients with B-ALL enrolled in COG AALL03B1 (2003–2011) for whom chromosome studies were available. Of 115 patients with hypodiploidy (25–39 chromosomes), karyotypes of 40 showed only the hypodiploid clone, 47 showed mosaicism with both hypodiploid and hyperdiploid (doubled) karyotypes, and 28 with masked hypodiploidy showed only a hyperdiploid (doubled) clone. Unique karyotypic signatures were identified, and widespread loss of heterozygosity (LOH) was seen in the microsatellite panel for all patients with masked hypodiploidy. An increased awareness of the unusual karyotypic profile associated with a doubled hypodiploid clone and coordinated use of DI, FISH, and LOH studies when indicated can identify patients with masked hypodiploidy and allow appropriate treatment selection.

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KW - Near-haploid

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