Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

M. Veta, A. Huisman, M. A. Viergever, P. J. Van Diest, J. P W Pluim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy.

Original languageEnglish (US)
Title of host publicationProceedings - International Symposium on Biomedical Imaging
Pages618-621
Number of pages4
DOIs
StatePublished - 2011
Externally publishedYes
Event2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11 - Chicago, IL, United States
Duration: Mar 30 2011Apr 2 2011

Other

Other2011 8th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI'11
CountryUnited States
CityChicago, IL
Period3/30/114/2/11

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Keywords

  • breast cancer
  • histology images
  • Marker-controlled watershed
  • nuclei segmentation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

Veta, M., Huisman, A., Viergever, M. A., Van Diest, P. J., & Pluim, J. P. W. (2011). Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. In Proceedings - International Symposium on Biomedical Imaging (pp. 618-621). [5872483] https://doi.org/10.1109/ISBI.2011.5872483