Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing

Bence Daniel, Balint L. Balint, Zsuzsanna S. Nagy, Laszlo Nagy

Research output: Contribution to journalArticlepeer-review

Abstract

Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modifi cation. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by “ChIP-grade” antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA- binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages.

Original languageEnglish (US)
Pages (from-to)15-24
Number of pages10
JournalMethods in Molecular Biology
Volume1204
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Binding site
  • ChIP
  • Chromatin
  • Cistrome
  • Macrophage
  • RXR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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