Mapping the functional yeast ABC transporter interactome

Jamie Snider, Asad Hanif, Mid Eum Lee, Ke Jin, Analyn R. Yu, Chris Graham, Matthew Chuk, Dunja Damjanovic, Marta Wierzbicka, Priscilla Tang, Dina Balderes, Victoria Wong, Matthew Jessulat, Katelyn D. Darowski, Bryan Joseph San Luis, Igor Shevelev, Stephen L. Sturley, Charles Boone, Jack F. Greenblatt, Zhaolei ZhangChristian M. Paumi, Mohan Babu, Hay Oak Park, Susan Michaelis, Igor Stagljar

Research output: Contribution to journalArticlepeer-review


ATP-binding cassette (ABC) transporters are a ubiquitous class of integral membrane proteins of immense clinical interest because of their strong association with human disease and pharmacology. To improve our understanding of these proteins, we used membrane yeast two-hybrid technology to map the protein interactome of all of the nonmitochondrial ABC transporters in the model organism Saccharomyces cerevisiae and combined this data with previously reported yeast ABC transporter interactions in the BioGRID database to generate a comprehensive, integrated 'interactome'. We show that ABC transporters physically associate with proteins involved in an unexpectedly diverse range of functions. We specifically examine the importance of the physical interactions of ABC transporters in both the regulation of one another and in the modulation of proteins involved in zinc homeostasis. The interaction network presented here will be a powerful resource for increasing our fundamental understanding of the cellular role and regulation of ABC transporters.

Original languageEnglish (US)
Pages (from-to)565-574
Number of pages10
JournalNature chemical biology
Issue number9
StatePublished - Sep 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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