Mapping the functional yeast ABC transporter interactome

Jamie Snider, Asad Hanif, Mid Eum Lee, Ke Jin, Analyn R. Yu, Chris Graham, Matthew Chuk, Dunja Damjanovic, Marta Wierzbicka, Priscilla Tang, Dina Balderes, Victoria Wong, Matthew Jessulat, Katelyn D. Darowski, Bryan Joseph San Luis, Igor Shevelev, Stephen L. Sturley, Charles Boone, Jack F. Greenblatt, Zhaolei ZhangChristian M. Paumi, Mohan Babu, Hay Oak Park, Susan Doris Michaelis, Igor Stagljar

Research output: Contribution to journalArticle

Abstract

ATP-binding cassette (ABC) transporters are a ubiquitous class of integral membrane proteins of immense clinical interest because of their strong association with human disease and pharmacology. To improve our understanding of these proteins, we used membrane yeast two-hybrid technology to map the protein interactome of all of the nonmitochondrial ABC transporters in the model organism Saccharomyces cerevisiae and combined this data with previously reported yeast ABC transporter interactions in the BioGRID database to generate a comprehensive, integrated 'interactome'. We show that ABC transporters physically associate with proteins involved in an unexpectedly diverse range of functions. We specifically examine the importance of the physical interactions of ABC transporters in both the regulation of one another and in the modulation of proteins involved in zinc homeostasis. The interaction network presented here will be a powerful resource for increasing our fundamental understanding of the cellular role and regulation of ABC transporters.

Original languageEnglish (US)
Pages (from-to)565-574
Number of pages10
JournalNature Chemical Biology
Volume9
Issue number9
DOIs
StatePublished - Sep 2013

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ATP-Binding Cassette Transporters
Yeasts
Membrane Proteins
Proteins
Saccharomyces cerevisiae
Zinc
Homeostasis
Databases
Pharmacology
Technology

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Snider, J., Hanif, A., Lee, M. E., Jin, K., Yu, A. R., Graham, C., ... Stagljar, I. (2013). Mapping the functional yeast ABC transporter interactome. Nature Chemical Biology, 9(9), 565-574. https://doi.org/10.1038/nchembio.1293

Mapping the functional yeast ABC transporter interactome. / Snider, Jamie; Hanif, Asad; Lee, Mid Eum; Jin, Ke; Yu, Analyn R.; Graham, Chris; Chuk, Matthew; Damjanovic, Dunja; Wierzbicka, Marta; Tang, Priscilla; Balderes, Dina; Wong, Victoria; Jessulat, Matthew; Darowski, Katelyn D.; San Luis, Bryan Joseph; Shevelev, Igor; Sturley, Stephen L.; Boone, Charles; Greenblatt, Jack F.; Zhang, Zhaolei; Paumi, Christian M.; Babu, Mohan; Park, Hay Oak; Michaelis, Susan Doris; Stagljar, Igor.

In: Nature Chemical Biology, Vol. 9, No. 9, 09.2013, p. 565-574.

Research output: Contribution to journalArticle

Snider, J, Hanif, A, Lee, ME, Jin, K, Yu, AR, Graham, C, Chuk, M, Damjanovic, D, Wierzbicka, M, Tang, P, Balderes, D, Wong, V, Jessulat, M, Darowski, KD, San Luis, BJ, Shevelev, I, Sturley, SL, Boone, C, Greenblatt, JF, Zhang, Z, Paumi, CM, Babu, M, Park, HO, Michaelis, SD & Stagljar, I 2013, 'Mapping the functional yeast ABC transporter interactome', Nature Chemical Biology, vol. 9, no. 9, pp. 565-574. https://doi.org/10.1038/nchembio.1293
Snider J, Hanif A, Lee ME, Jin K, Yu AR, Graham C et al. Mapping the functional yeast ABC transporter interactome. Nature Chemical Biology. 2013 Sep;9(9):565-574. https://doi.org/10.1038/nchembio.1293
Snider, Jamie ; Hanif, Asad ; Lee, Mid Eum ; Jin, Ke ; Yu, Analyn R. ; Graham, Chris ; Chuk, Matthew ; Damjanovic, Dunja ; Wierzbicka, Marta ; Tang, Priscilla ; Balderes, Dina ; Wong, Victoria ; Jessulat, Matthew ; Darowski, Katelyn D. ; San Luis, Bryan Joseph ; Shevelev, Igor ; Sturley, Stephen L. ; Boone, Charles ; Greenblatt, Jack F. ; Zhang, Zhaolei ; Paumi, Christian M. ; Babu, Mohan ; Park, Hay Oak ; Michaelis, Susan Doris ; Stagljar, Igor. / Mapping the functional yeast ABC transporter interactome. In: Nature Chemical Biology. 2013 ; Vol. 9, No. 9. pp. 565-574.
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