Mapping the amphetamine-evoked dopamine release in the brain of the Göttingen minipig

Nanna Marie Lind, Aage Kristian Olsen, Anette Moustgaard, Svend Borup Jensen, Steen Jakobsen, Axel Kornerup Hansen, Sidse Marie Arnfred, Ralf Peter Hemmingsen, Albert Gjedde, Paul Cumming

Research output: Contribution to journalArticle

Abstract

The availability of dopamine D2/3 binding sites in brain of six male and six female Göttingen minipigs was measured in a baseline condition and after challenge with amphetamine sulfate (1 mg/kg, i.v.) in PET studies with [11C]raclopride. Maps of the binding potential (pB; B max/Kd) of [11C]raclopride were spatially normalized and co-registered to a common stereotaxic coordinate system for pig brain. The pB maps were then analyzed by volume of interest and voxel-wise comparisons of gender and condition. The mean baseline pB tended to be 10-20% higher in striatum of the female group, but this gender difference was not significant. Variance of the mean baseline pB was higher in the males (44%) than in females (30%), but there was no correlation between pB and individual plasma cortisol or testosterone concentrations. Using statistical parametric mapping, we detected a focus in the right posterior putamen where the magnitude of the amphetamine-evoked decrease in pB was greater in the male than in the female group. Thus, the spatial pattern of reactivity of dopamine D2/3 receptor availability to amphetamine challenge is not identical in male and female pigs. Within the entire population, the decline in pB evoked by amphetamine (ΔpB) was greater in the ventral striatum (-28%) than in the caudate nucleus (-17%), consistent with earlier reports in monkeys and humans. The magnitude of ΔpB correlated highly with the baseline pB values in all divisions of the striatum. Based upon the principles of competitive binding, the slope of this empirical relationship, fi, is equal to the fraction of [11C]raclopride binding sites sensitive to endogenous dopamine; the magnitude of this fraction ranged from 0.29 in the caudate to 0.36 in the ventral striatum.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalBrain Research Bulletin
Volume65
Issue number1
DOIs
StatePublished - Feb 15 2005
Externally publishedYes

Fingerprint

Miniature Swine
Amphetamine
Dopamine
Raclopride
Brain
Swine
Binding Sites
Competitive Binding
Dopamine D2 Receptors
Caudate Nucleus
Putamen
Haplorhini
Hydrocortisone
Testosterone
Population

Keywords

  • [C]raclopride
  • Amphetamine
  • Cortisol
  • Dopamine receptors
  • Pig

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lind, N. M., Olsen, A. K., Moustgaard, A., Jensen, S. B., Jakobsen, S., Hansen, A. K., ... Cumming, P. (2005). Mapping the amphetamine-evoked dopamine release in the brain of the Göttingen minipig. Brain Research Bulletin, 65(1), 1-9. https://doi.org/10.1016/j.brainresbull.2004.08.007

Mapping the amphetamine-evoked dopamine release in the brain of the Göttingen minipig. / Lind, Nanna Marie; Olsen, Aage Kristian; Moustgaard, Anette; Jensen, Svend Borup; Jakobsen, Steen; Hansen, Axel Kornerup; Arnfred, Sidse Marie; Hemmingsen, Ralf Peter; Gjedde, Albert; Cumming, Paul.

In: Brain Research Bulletin, Vol. 65, No. 1, 15.02.2005, p. 1-9.

Research output: Contribution to journalArticle

Lind, NM, Olsen, AK, Moustgaard, A, Jensen, SB, Jakobsen, S, Hansen, AK, Arnfred, SM, Hemmingsen, RP, Gjedde, A & Cumming, P 2005, 'Mapping the amphetamine-evoked dopamine release in the brain of the Göttingen minipig', Brain Research Bulletin, vol. 65, no. 1, pp. 1-9. https://doi.org/10.1016/j.brainresbull.2004.08.007
Lind, Nanna Marie ; Olsen, Aage Kristian ; Moustgaard, Anette ; Jensen, Svend Borup ; Jakobsen, Steen ; Hansen, Axel Kornerup ; Arnfred, Sidse Marie ; Hemmingsen, Ralf Peter ; Gjedde, Albert ; Cumming, Paul. / Mapping the amphetamine-evoked dopamine release in the brain of the Göttingen minipig. In: Brain Research Bulletin. 2005 ; Vol. 65, No. 1. pp. 1-9.
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