The simian virus 40 (SV40) DNA segment in the nondefective adenovirus 2 SV40 hybrid, Ad2+ND4, is colinear with the segment between 0.11 and 0.59 SV40 fractional length from the site at which the R1 restriction endonuclease cleaves SV40 DNA. This specifies the region of the SV40 DNA molecule which induces the early SV40 antigens: U antigen, tumor specific transplantation antigen, and T antigen. A variant of Ad2+ND4, called Ad2+ND(4del), was found which has a deletion of the DNA segment between 0.50 and 0.57 SV40 fractional length from the R1 endonuclease cleavage point.
ASJC Scopus subject areas
- Insect Science