Mapping of noninvasion TnphoA mutations on the escherichia coli O18:K1:H7 chromosome

Craig A. Bloch, Sheng He Huang, Christopher K. Rode, Kwang Sik Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The most virulent newborn meningitis-associated Escherichia coli are of the serotype O18:K1:H7. We previously isolated a large number of E. coli 018:K1:H7 mutants resulting from transposon TnphoA mutagenesis that-fail to invade brain microvascular endothelial cells. We have now determined the locations of 45 independent insertions. Twelve were localized to the 98 min region, containing a 120 kb segment that is characteristic of E. coli 018:K1:H7. Another, the previously described insertion ibe-10::TnphoA, was localized to the 87 min region, containing a 20 kb segment found in this E. coli. These noninvasion mutations may define new O18:K1:H17 pathogenicity islands carrying genes for penetration of the blood-brain barrier of newborn mammals.

Original languageEnglish (US)
Pages (from-to)171-176
Number of pages6
JournalFEMS microbiology letters
Volume144
Issue number2-3
DOIs
StatePublished - Nov 1 1996
Externally publishedYes

Keywords

  • Bacterial genomics
  • Escherichia coli meningitis
  • I-SceI restriction endonuclease
  • Pathogenicity island
  • Pulsed-field gel electrophoresis
  • Rare-restriction-site mapping

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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