Mapping ischemic risk region and necrosis in the isolated heart using EPR imaging

Murugesan Velayutham, Haiquan Li, Periannan Kuppusamy, Jay L. Zweier

Research output: Contribution to journalArticle

Abstract

Reperfusion of ischemic tissue is a common event in the treatment of heart attack and stroke. To study disease pathogenesis, methods are required to measure tissue perfusion and area at risk, as well as localized regions of injury. While histology can provide this information, its destructive nature precludes assessment of time course. Thus, there is a critical need for a noninvasive technique to obtain this information. To map myocardial redox state as a possible index of cellular ischemia and viability, electron paramagnetic resonance (EPR) imaging experiments were performed on isolated rat hearts before and after the onset of regional ischemia using nitroxide spin labels. With coronary artery occlusion, the EPR images clearly showed the risk region as a void of lower intensity that reversed upon reperfusion. The extent of risk region in the heart was similar in EPR imaging and histological measurements. The unique information obtained regarding the time course of changes in redox metabolism of the risk region and normal myocardium can provide important insights regarding the mechanisms of myocardial injury during and following ischemia.

Original languageEnglish (US)
Pages (from-to)1181-1187
Number of pages7
JournalMagnetic Resonance in Medicine
Volume49
Issue number6
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Keywords

  • Cell death
  • EPR imaging
  • Nitroxide
  • Rat heart
  • Regional ischemia

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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