Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily

M. Dean, R. Allikmets, B. Gerrard, C. Stewart, A. Kistler, B. Shafer, Susan Doris Michaelis, J. Strathern

Research output: Contribution to journalArticle

Abstract

ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).

Original languageEnglish (US)
Pages (from-to)377-383
Number of pages7
JournalYeast
Volume10
Issue number3
StatePublished - 1994

Fingerprint

Adenosinetriphosphate
Yeast
Genes
Adenosine Triphosphate
Yeasts
yeasts
genes
Multiple Drug Resistance
multiple drug resistance
peptide transporters
ATP-Binding Cassette Transporters
Essential Genes
Nucleic Acid Databases
Glycoproteins
loci
P-Glycoprotein
ABC transporters
Oligonucleotides
Chromosomes
oligonucleotides

Keywords

  • ABC gene
  • chromosome XII
  • chromosome XVI
  • Multidrug resistance

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Dean, M., Allikmets, R., Gerrard, B., Stewart, C., Kistler, A., Shafer, B., ... Strathern, J. (1994). Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily. Yeast, 10(3), 377-383.

Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily. / Dean, M.; Allikmets, R.; Gerrard, B.; Stewart, C.; Kistler, A.; Shafer, B.; Michaelis, Susan Doris; Strathern, J.

In: Yeast, Vol. 10, No. 3, 1994, p. 377-383.

Research output: Contribution to journalArticle

Dean, M, Allikmets, R, Gerrard, B, Stewart, C, Kistler, A, Shafer, B, Michaelis, SD & Strathern, J 1994, 'Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily', Yeast, vol. 10, no. 3, pp. 377-383.
Dean M, Allikmets R, Gerrard B, Stewart C, Kistler A, Shafer B et al. Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily. Yeast. 1994;10(3):377-383.
Dean, M. ; Allikmets, R. ; Gerrard, B. ; Stewart, C. ; Kistler, A. ; Shafer, B. ; Michaelis, Susan Doris ; Strathern, J. / Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily. In: Yeast. 1994 ; Vol. 10, No. 3. pp. 377-383.
@article{5dae31df80074a76b6963fb4032b2249,
title = "Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily",
abstract = "ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).",
keywords = "ABC gene, chromosome XII, chromosome XVI, Multidrug resistance",
author = "M. Dean and R. Allikmets and B. Gerrard and C. Stewart and A. Kistler and B. Shafer and Michaelis, {Susan Doris} and J. Strathern",
year = "1994",
language = "English (US)",
volume = "10",
pages = "377--383",
journal = "Yeast",
issn = "0749-503X",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Mapping and sequencing of two yeast genes belonging to the ATP-binding cassette superfamily

AU - Dean, M.

AU - Allikmets, R.

AU - Gerrard, B.

AU - Stewart, C.

AU - Kistler, A.

AU - Shafer, B.

AU - Michaelis, Susan Doris

AU - Strathern, J.

PY - 1994

Y1 - 1994

N2 - ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).

AB - ATP-binding cassette (ABC) transporters share significant sequence identity within their ATP-binding domains. Degenerate oligonucleotides based on highly conserved portions of the ATP-binding domain genes were used to clone portions of two members of the ABC gene superfamily from Saccharomyces cerevisiae DNA. These genes were designated MDL1 and MDL2 (for multidrug resistance-like). Each MDL gene is predicted to encode a single set of transmembrane domains and a single ATP-binding domain, thus the MDL gene products are 'half-molecule' ABC proteins. The two genes were mapped to precise regions on chromosomes XII and XVI and show a considerable similarity to the mammalian P-glycoprotein/multidrug resistance (MDR) and peptide transporter (TAP) genes. Preliminary analysis of null mutants constructed by gene replacement has indicated that the MDL genes are not essential for viability of yeast. The sequences have been deposited in the GenBank data library under Accession Numbers L16958 (Locus YSCBCSA) and L16959 (Locus YSCBCSB).

KW - ABC gene

KW - chromosome XII

KW - chromosome XVI

KW - Multidrug resistance

UR - http://www.scopus.com/inward/record.url?scp=0028278937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028278937&partnerID=8YFLogxK

M3 - Article

C2 - 7912468

AN - SCOPUS:0028278937

VL - 10

SP - 377

EP - 383

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 3

ER -