TY - JOUR
T1 - Mapping and characterization of the primary and anamnestic H-2 d-restricted cytotoxic T-lymphocyte response in mice against human metapneumovirus
AU - Melendi, Guillermina A.
AU - Zavala, Fidel
AU - Buchholz, Ursula J.
AU - Boivin, Guy
AU - Collins, Peter L.
AU - Kleeberger, Steven R.
AU - Polack, Fernando P.
PY - 2007/10
Y1 - 2007/10
N2 - Cytotoxic T lymphocytes (CTLs) are important for the control of virus replication during respiratory infections. For human metapneumovirus (hMPV), an H-2d-restricted CTL epitope in the M2-2 protein has been described. In this study, we screened the hMPV F, G, N, M, M2-1, and M2-2 proteins using three independent algorithms to predict H-2d CTL epitopes in BALB/c mice. A dominant epitope (GYIDDNQSI) in positions 81 to 89 of the antitermination factor M2-1 and a subdominant epitope (SPKAGLLSL) in N 307-315 were detected during the anti-hMPV CTL response. Passive transfer of CD8+ T-cell lines against M2-181-89 and N 307-315 protected Rag1-/- mice against hMPV challenge. Interestingly, diversification of CTL targets to include multiple epitopes was observed after repetitive infections. A subdominant response against the previously described M2-2 epitope was detected after the third infection. An understanding of the CTL response against hMPV is important for developing preventive and therapeutic strategies against the virus.
AB - Cytotoxic T lymphocytes (CTLs) are important for the control of virus replication during respiratory infections. For human metapneumovirus (hMPV), an H-2d-restricted CTL epitope in the M2-2 protein has been described. In this study, we screened the hMPV F, G, N, M, M2-1, and M2-2 proteins using three independent algorithms to predict H-2d CTL epitopes in BALB/c mice. A dominant epitope (GYIDDNQSI) in positions 81 to 89 of the antitermination factor M2-1 and a subdominant epitope (SPKAGLLSL) in N 307-315 were detected during the anti-hMPV CTL response. Passive transfer of CD8+ T-cell lines against M2-181-89 and N 307-315 protected Rag1-/- mice against hMPV challenge. Interestingly, diversification of CTL targets to include multiple epitopes was observed after repetitive infections. A subdominant response against the previously described M2-2 epitope was detected after the third infection. An understanding of the CTL response against hMPV is important for developing preventive and therapeutic strategies against the virus.
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U2 - 10.1128/JVI.02423-06
DO - 10.1128/JVI.02423-06
M3 - Article
C2 - 17670840
AN - SCOPUS:35148884680
SN - 0022-538X
VL - 81
SP - 11461
EP - 11467
JO - Journal of virology
JF - Journal of virology
IS - 20
ER -