Manipulation of the immune response by foreign gene expression in the thymus

D. J. Marshall, B. H. Park, J. M. Korostoff, G. N. Gaulton

Research output: Contribution to journalArticlepeer-review

Abstract

Retroviral gene transfer vectors have been developed for optimal in vivo gene therapy. Ideally, these vectors should target gene expression specifically to selected tissues or organs. Our studies focus on the development of retroviral vectors for gene delivery to the thymus. The goal of these studies is to utilize thymic expression of exogenous genes to manipulate the immune repertoire. We have characterized the selective thymic tropism of a molecular clone of Gross murine leukemia virus, GD-17, to thymic medullary epithelial cells using immunohistochemical staining and confocal microscopy. Specific expression of viral antigens In the thymus lead to the induction of immunologic tolerance to GMuLV proteins. This tissue specific vector may thus be used to study the requirements of epithelial mediated tolerance induction, and provide a more efficient tool for gene therapy.

Original languageEnglish (US)
JournalLeukemia
Volume9
Issue numberSUPPL. 1
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

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