Mammalian orthologues of a yeast regulator of nonsense transcript stability

Haley A. Perlick, Susan M. Medghalchi, Forrest A. Spencer, Raymond J. Kendzior, Harry C. Dietz

Research output: Contribution to journalArticle

Abstract

All eukaryotes that have been studied to date possess the ability to detect and degrade transcripts that contain a premature signal for the termination of translation. This process of nonsense-mediated RNA decay has been most comprehensively studied in the yeast Saccharomyces cerevisiae where at least three trans-acting factors (Upf1p through Upf3p) are required. We have cloned cDNAs encoding human and murine homologues of Upf1p, termed rent1 (regulator of nonsense transcripts). Rent1 is the first identified mammalian protein that contains all of the putative functional elements in Upf1p including zinc finger-like and NTPase domains, as well as all motifs common to members of helicase superfamily 1. Moreover, expression of a chimeric protein, containing the central region of rent1 flanked by the extreme N and C termini of Upf1p, complements the Upf1p-deficient phenotype in yeast. Thus, despite apparent differences between yeast and mammalian nonsense-mediated RNA decay, these data suggest that the two pathways use functionally related machinery.

Original languageEnglish (US)
Pages (from-to)10928-10932
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number20
DOIs
StatePublished - Oct 1 1996

Keywords

  • RNA degradation
  • RNA metabolism
  • nonsense mutation
  • premature termination codon

ASJC Scopus subject areas

  • General

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