Mammalian Auditory Hair Cell Bundle Stiffness Affects Frequency Tuning by Increasing Coupling along the Length of the Cochlea

James B. Dewey, Anping Xia, Ulrich Mueller, Inna A. Belyantseva, Brian E. Applegate, John S. Oghalai

Research output: Contribution to journalArticle

Abstract

The stereociliary bundles of cochlear hair cells convert mechanical vibrations into the electrical signals required for auditory sensation. While the stiffness of the bundles strongly influences mechanotransduction, its influence on the vibratory response of the cochlear partition is unclear. To assess this, we measured cochlear vibrations in mutant mice with reduced bundle stiffness or with a tectorial membrane (TM) that is detached from the sensory epithelium. We found that reducing bundle stiffness decreased the high-frequency extent and sharpened the tuning of vibratory responses obtained postmortem. Detaching the TM further reduced the high-frequency extent of the vibrations but also lowered the partition's resonant frequency. Together, these results demonstrate that the bundle's stiffness and attachment to the TM contribute to passive longitudinal coupling in the cochlea. We conclude that the stereociliary bundles and TM interact to facilitate passive-wave propagation to more apical locations, possibly enhancing active-wave amplification in vivo. The mechanical properties of the cochlear partition determine its vibratory response to sound. Dewey et al. demonstrate that the outer hair cell stereociliary bundles’ stiffness and attachment to the tectorial membrane influence the partition's passive vibratory response. The stereociliary bundles facilitate the propagation of cochlear waves to more apical regions.

Original languageEnglish (US)
Pages (from-to)2915-2927
Number of pages13
JournalCell Reports
Volume23
Issue number10
DOIs
StatePublished - Jun 5 2018

Fingerprint

Auditory Hair Cells
Tectorial Membrane
Cochlea
Tuning
Cells
Stiffness
Membranes
Vibration
Outer Auditory Hair Cells
Wave propagation
Vibrations (mechanical)
Amplification
Natural frequencies
Epithelium
Acoustic waves
Mechanical properties

Keywords

  • cochlear mechanics
  • hair cell stereocilia
  • optical coherence tomography
  • tectorial membrane

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mammalian Auditory Hair Cell Bundle Stiffness Affects Frequency Tuning by Increasing Coupling along the Length of the Cochlea. / Dewey, James B.; Xia, Anping; Mueller, Ulrich; Belyantseva, Inna A.; Applegate, Brian E.; Oghalai, John S.

In: Cell Reports, Vol. 23, No. 10, 05.06.2018, p. 2915-2927.

Research output: Contribution to journalArticle

Dewey, James B. ; Xia, Anping ; Mueller, Ulrich ; Belyantseva, Inna A. ; Applegate, Brian E. ; Oghalai, John S. / Mammalian Auditory Hair Cell Bundle Stiffness Affects Frequency Tuning by Increasing Coupling along the Length of the Cochlea. In: Cell Reports. 2018 ; Vol. 23, No. 10. pp. 2915-2927.
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