Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene

A. Kovnat, R. N. Buick, B. Choo, E. De Harven, I. Kopelyan, J. M. Trent, I. F. Tannock

Research output: Contribution to journalArticle

Abstract

The human bladder cancer cell line MGH-U1 (also designated T-24 or EJ) contains an activated c-Ha-ras oncogene, which is amplified as compared to normal human fibroblasts. We have generated sublines from the MGH-U1 cell line: the MGH-U1/OCI subline was generated by dissociating spheroids formed from MGH-U1 cells; the U1-m/F1 and OCI-m/F1 were generated by in vivo passage of experimental lung metastases formed after i.v. injection of MGH-U1 and MGH-U1/OCI lines into immune-deprived mice; the U1/t subline was generated by in vitro passage of i.m. tumors formed from MGH-U1 cells. All sublines formed tumors in immune-deprived mice from smaller i.m. inocula than the parent line, and the U1-m/F1 subline generated more spontaneous metastases in lungs. Lung colony forming efficiency after i.v. injections of cells into similar mice was also greater for the sublines than for the parent MGH-U1 cells. The U1-m/F1 and OCI-m/F1 were the most tumorigenic lines. Early passages of the MGH-U1/OCI subline showed the presence of double minute chromosomes, and amplification and increased expression of the c-Ha-ras oncogene as compared to the parental cell line. These changes were not present in later cultures of MGH-U1/OCI cells, and no consistent difference in the levels of gene amplification or expression between the parent line and the sublines was found. Thus the content and expression of the activated c-Ha-ras oncogene does not correlate with malignant properties of the sublines.

Original languageEnglish (US)
Pages (from-to)4993-5000
Number of pages8
JournalCancer Research
Volume48
Issue number17
StatePublished - 1988
Externally publishedYes

Fingerprint

ras Genes
Urinary Bladder Neoplasms
Cell Line
Lung
Neoplasm Metastasis
Injections
Gene Amplification
Neoplasms
Fibroblasts
Chromosomes
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kovnat, A., Buick, R. N., Choo, B., De Harven, E., Kopelyan, I., Trent, J. M., & Tannock, I. F. (1988). Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene. Cancer Research, 48(17), 4993-5000.

Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene. / Kovnat, A.; Buick, R. N.; Choo, B.; De Harven, E.; Kopelyan, I.; Trent, J. M.; Tannock, I. F.

In: Cancer Research, Vol. 48, No. 17, 1988, p. 4993-5000.

Research output: Contribution to journalArticle

Kovnat, A, Buick, RN, Choo, B, De Harven, E, Kopelyan, I, Trent, JM & Tannock, IF 1988, 'Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene', Cancer Research, vol. 48, no. 17, pp. 4993-5000.
Kovnat A, Buick RN, Choo B, De Harven E, Kopelyan I, Trent JM et al. Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene. Cancer Research. 1988;48(17):4993-5000.
Kovnat, A. ; Buick, R. N. ; Choo, B. ; De Harven, E. ; Kopelyan, I. ; Trent, J. M. ; Tannock, I. F. / Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-Ha-ras oncogene. In: Cancer Research. 1988 ; Vol. 48, No. 17. pp. 4993-5000.
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abstract = "The human bladder cancer cell line MGH-U1 (also designated T-24 or EJ) contains an activated c-Ha-ras oncogene, which is amplified as compared to normal human fibroblasts. We have generated sublines from the MGH-U1 cell line: the MGH-U1/OCI subline was generated by dissociating spheroids formed from MGH-U1 cells; the U1-m/F1 and OCI-m/F1 were generated by in vivo passage of experimental lung metastases formed after i.v. injection of MGH-U1 and MGH-U1/OCI lines into immune-deprived mice; the U1/t subline was generated by in vitro passage of i.m. tumors formed from MGH-U1 cells. All sublines formed tumors in immune-deprived mice from smaller i.m. inocula than the parent line, and the U1-m/F1 subline generated more spontaneous metastases in lungs. Lung colony forming efficiency after i.v. injections of cells into similar mice was also greater for the sublines than for the parent MGH-U1 cells. The U1-m/F1 and OCI-m/F1 were the most tumorigenic lines. Early passages of the MGH-U1/OCI subline showed the presence of double minute chromosomes, and amplification and increased expression of the c-Ha-ras oncogene as compared to the parental cell line. These changes were not present in later cultures of MGH-U1/OCI cells, and no consistent difference in the levels of gene amplification or expression between the parent line and the sublines was found. Thus the content and expression of the activated c-Ha-ras oncogene does not correlate with malignant properties of the sublines.",
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AU - Kovnat, A.

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AU - De Harven, E.

AU - Kopelyan, I.

AU - Trent, J. M.

AU - Tannock, I. F.

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N2 - The human bladder cancer cell line MGH-U1 (also designated T-24 or EJ) contains an activated c-Ha-ras oncogene, which is amplified as compared to normal human fibroblasts. We have generated sublines from the MGH-U1 cell line: the MGH-U1/OCI subline was generated by dissociating spheroids formed from MGH-U1 cells; the U1-m/F1 and OCI-m/F1 were generated by in vivo passage of experimental lung metastases formed after i.v. injection of MGH-U1 and MGH-U1/OCI lines into immune-deprived mice; the U1/t subline was generated by in vitro passage of i.m. tumors formed from MGH-U1 cells. All sublines formed tumors in immune-deprived mice from smaller i.m. inocula than the parent line, and the U1-m/F1 subline generated more spontaneous metastases in lungs. Lung colony forming efficiency after i.v. injections of cells into similar mice was also greater for the sublines than for the parent MGH-U1 cells. The U1-m/F1 and OCI-m/F1 were the most tumorigenic lines. Early passages of the MGH-U1/OCI subline showed the presence of double minute chromosomes, and amplification and increased expression of the c-Ha-ras oncogene as compared to the parental cell line. These changes were not present in later cultures of MGH-U1/OCI cells, and no consistent difference in the levels of gene amplification or expression between the parent line and the sublines was found. Thus the content and expression of the activated c-Ha-ras oncogene does not correlate with malignant properties of the sublines.

AB - The human bladder cancer cell line MGH-U1 (also designated T-24 or EJ) contains an activated c-Ha-ras oncogene, which is amplified as compared to normal human fibroblasts. We have generated sublines from the MGH-U1 cell line: the MGH-U1/OCI subline was generated by dissociating spheroids formed from MGH-U1 cells; the U1-m/F1 and OCI-m/F1 were generated by in vivo passage of experimental lung metastases formed after i.v. injection of MGH-U1 and MGH-U1/OCI lines into immune-deprived mice; the U1/t subline was generated by in vitro passage of i.m. tumors formed from MGH-U1 cells. All sublines formed tumors in immune-deprived mice from smaller i.m. inocula than the parent line, and the U1-m/F1 subline generated more spontaneous metastases in lungs. Lung colony forming efficiency after i.v. injections of cells into similar mice was also greater for the sublines than for the parent MGH-U1 cells. The U1-m/F1 and OCI-m/F1 were the most tumorigenic lines. Early passages of the MGH-U1/OCI subline showed the presence of double minute chromosomes, and amplification and increased expression of the c-Ha-ras oncogene as compared to the parental cell line. These changes were not present in later cultures of MGH-U1/OCI cells, and no consistent difference in the levels of gene amplification or expression between the parent line and the sublines was found. Thus the content and expression of the activated c-Ha-ras oncogene does not correlate with malignant properties of the sublines.

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