Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-ha-ras oncogene

A. Kovnat, R. N. Buick, B. Choo, E. De Harven, I. Kopelyan, J. M. Trent, I. F. Tannock

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Abstract

The human bladder cancer cell line MGH-U1 (also designated T-24 or EJ) contains an activated c-Ha-ras oncogene, which is amplified as compared to normal human fibroblasts. We have generated sublines from the MGH-U1 cell line: The MGH-U1/OCI subline was generated by dissociating spheroids formed from MGH-U1 cells; the Ul-m/F1 and OCI-m/Fi were generated by in vivo passage of experimental lung metastases formed after i.v. injection of MGH-U1 and MGH-U1/OCI lines into immune-deprived mice; the Ul/t subline was generated by in vivo passage of i.m. tumors formed from MGH-U1 cells. All sublines formed tumors in immune-deprived mice from smaller i.m. inocula than the parent line, and the Ul-m/F1 subline generated more spontaneous metastases in lungs. Lung colony forming efficiency after i.v. injections of cells into similar mice was. also greater for the sublines than for the parent MGH-U1 cells. The Ul-m/F1 and OCl-m/F1 were the most tumorigenic lines. Early passages of the MGH-U1/OCI subline showed the presence of double minute chromosomes, and amplification and increased expression of the c-Ha-ras oncogene as compared to the parental cell line. These changes were not present in later cultures of MGH-U1/OCI cells, and no consistent difference in the levels of gene amplification or expression between the parent line and the sublines was found. Thus the content and expression of the activated c-Ha-ras oncogene does not correlate with malignant properties of the sublines.

Original languageEnglish (US)
Pages (from-to)4993-5000
Number of pages8
JournalCancer Research
Volume48
Issue number17
StatePublished - Sep 1 1988

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kovnat, A., Buick, R. N., Choo, B., De Harven, E., Kopelyan, I., Trent, J. M., & Tannock, I. F. (1988). Malignant properties of sublines selected from a human bladder cancer cell line that contains an activated c-ha-ras oncogene. Cancer Research, 48(17), 4993-5000.