Malignant cells derived from 3T3 fibroblast feeder layer in cell culture for nasopharyngeal carcinoma

Fenggang Yu, Wen son Hsieh, Fredrik Petersson, Henry Yang, Yingying Li, Chunwei Li, Soon Wah Low, Jing Liu, Yan Yan, De Yun Wang, Kwok Seng Loh

Research output: Contribution to journalArticle

Abstract

In this study, we discovered a subpopulation of 3T3 feeder cells were malignantly transformed by nasopharyngeal carcinoma (NPC) tumor cells during co-culture. The transformed 3T3 cells acquired an accelerated growth rate, displayed loosely attached multilayer growth in vitro and highly tumorigenic in vivo. Most strikingly, instead of forming sarcomas, they developed into carcinoma-like tumors somewhat resembling the original NPC. We further demonstrated the transformation is not a single isolated event, rather a common reproducible, cell contact dispensable phenomena among NPC tumor cells. However, NPC tumor cells alone were not sufficient to confer the transformed characteristics onto normal human cells.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalExperimental Cell Research
Volume322
Issue number1
DOIs
StatePublished - Mar 10 2014
Externally publishedYes

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Keywords

  • 3T3 cells
  • Cell contact
  • Feeder layer
  • Nasopharyngeal carcinoma
  • Transform

ASJC Scopus subject areas

  • Cell Biology

Cite this

Yu, F., Hsieh, W. S., Petersson, F., Yang, H., Li, Y., Li, C., Low, S. W., Liu, J., Yan, Y., Wang, D. Y., & Loh, K. S. (2014). Malignant cells derived from 3T3 fibroblast feeder layer in cell culture for nasopharyngeal carcinoma. Experimental Cell Research, 322(1), 193-201. https://doi.org/10.1016/j.yexcr.2013.12.015