Malignant and nonmalignant T cell lines from human T cell lymphotropic virus type I-negative patients with Sézary syndrome

J. Todd Abrams, Stuart R. Lessin, Subrata K. Ghosh, Peter C. Nowell, William Ju, Eric C. Vonderheid, Alain H. Rook, Elaine Defreitas

Research output: Contribution to journalArticle

Abstract

The establishment of an in vitro model for cutaneous T cell lymphomas and Sézary syndrome has been difficult since T cells from individuals with these diseases do not proliferate in response to T cell mitogens. We found that conditioned media, collected from mitogen-activated PBMC from Sézary patients, contain an IL-2 receptor inducing factor. Despite their ostensible proliferative disorder, using a combination Sézary cell-conditioned media and rIL-2, we established IL-2 responsive, human T cell lymphotropic virus type I negative T cell lines from 23 patients, nine of which contain cells with the structural and/or genetic characteristics of neoplastic Sézary T cells.

Original languageEnglish (US)
Pages (from-to)1455-1462
Number of pages8
JournalJournal of Immunology
Volume146
Issue number5
StatePublished - Mar 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Abrams, J. T., Lessin, S. R., Ghosh, S. K., Nowell, P. C., Ju, W., Vonderheid, E. C., Rook, A. H., & Defreitas, E. (1991). Malignant and nonmalignant T cell lines from human T cell lymphotropic virus type I-negative patients with Sézary syndrome. Journal of Immunology, 146(5), 1455-1462.