Male Mouse Accessory Sex Glands in Organ Culture

E. Spring-Mills, M. Bush, C. Hill

Research output: Contribution to journalArticle

Abstract

Explants of epididymis, vas deferens, seminal vesicle, and coagulating gland from 10-1 2-week old and 7-8-month-old male Swiss Webster mice were maintained in synthetic media without serum or hormones for up to 28 days. Differences were observed in the survival and responses of each gland and the behavior of the principal and basal cells. Epithelial cell migration was pronounced in the vas, moderate in the epididymis, and low in the seminal vesicle and coagulating gland. The incidence of basal cell proliferation, hyperplasia, and squamous metaplasia usually was positively related to the frequency of basal cells in the zero-time explants. Few differences were observed between the survival or responses of explants from young and old animals; however, explant culture appears useful for exploring fundamental properties of each accessory sex gland, especially the properties of basal cells.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalArchives of Andrology
Volume10
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Organ Culture Techniques
Seminal Vesicles
Epididymis
Vas Deferens
Metaplasia
Cell Movement
Hyperplasia
Epithelial Cells
Cell Proliferation
Hormones
Incidence
Serum

Keywords

  • Accessory Sex Glands
  • Aging
  • Coagulating Gland
  • Epididymis
  • Hyperplasia
  • Metaplasia
  • Organ Culture
  • Proliferation
  • Seminal Vesicle
  • Vas Deferens

ASJC Scopus subject areas

  • Reproductive Medicine
  • Endocrinology, Diabetes and Metabolism
  • Urology
  • Endocrinology

Cite this

Male Mouse Accessory Sex Glands in Organ Culture. / Spring-Mills, E.; Bush, M.; Hill, C.

In: Archives of Andrology, Vol. 10, No. 2, 1983, p. 103-111.

Research output: Contribution to journalArticle

Spring-Mills, E, Bush, M & Hill, C 1983, 'Male Mouse Accessory Sex Glands in Organ Culture', Archives of Andrology, vol. 10, no. 2, pp. 103-111. https://doi.org/10.3109/01485018308987550
Spring-Mills, E. ; Bush, M. ; Hill, C. / Male Mouse Accessory Sex Glands in Organ Culture. In: Archives of Andrology. 1983 ; Vol. 10, No. 2. pp. 103-111.
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