MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model

Lu Jiang, Kamila Chughtai, Samuel O. Purvine, Zaver M Bhujwalla, Venu Raman, Ljiljana Paša-Tolić, Ron M A Heeren, Kristine Glunde

Research output: Contribution to journalArticle

Abstract

Hypoxic areas are a common feature of rapidly growing malignant tumors and their metastases and are typically spatially heterogeneous. Hypoxia has a strong impact on tumor cell biology and contributes to tumor progression in multiple ways. To date, only a few molecular key players in tumor hypoxia, such as hypoxia-inducible factor-1 (HIF-1), have been discovered. The distribution of biomolecules is frequently heterogeneous in the tumor volume and may be driven by hypoxia and HIF-1α. Understanding the spatially heterogeneous hypoxic response of tumors is critical. Mass spectrometric imaging (MSI) provides a unique way of imaging biomolecular distributions in tissue sections with high spectral and spatial resolution. In this paper, breast tumor xenografts grown from MDA-MB-231-HRE-tdTomato cells, with a red fluorescent tdTomato protein construct under the control of a hypoxia response element (HRE)-containing promoter driven by HIF-1α, were used to detect the spatial distribution of hypoxic regions. We elucidated the 3D spatial relationship between hypoxic regions and the localization of lipids and proteins by using principal component analysis-linear discriminant analysis (PCA-LDA) on 3D rendered MSI volume data from MDA-MB-231-HRE-tdTomato breast tumor xenografts. In this study, we identified hypoxia-regulated proteins active in several distinct pathways such as glucose metabolism, regulation of actin cytoskeleton, protein folding, translation/ribosome, splicesome, the PI3K-Akt signaling pathway, hemoglobin chaperone, protein processing in endoplasmic reticulum, detoxification of reactive oxygen species, aurora B signaling/apoptotic execution phase, the RAS signaling pathway, the FAS signaling pathway/caspase cascade in apoptosis, and telomere stress induced senescence. In parallel, we also identified colocalization of hypoxic regions and various lipid species such as PC(16:0/18:0), PC(16:0/18:1), PC(16:0/18:2), PC(16:1/18:4), PC(18:0/18:1), and PC(18:1/18:1), among others. Our findings shed light on the biomolecular composition of hypoxic tumor regions, which may be responsible for a given tumors resistance to radiation or chemotherapy.

Original languageEnglish (US)
Pages (from-to)5947-5956
Number of pages10
JournalAnalytical Chemistry
Volume87
Issue number12
DOIs
StatePublished - Jun 16 2015

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tumors
Breast Neoplasms
Lipids
Imaging techniques
Hypoxia-Inducible Factor 1
Response Elements
Neoplasms
Proteins
Heterografts
Protein Folding
Telomere
Protein Biosynthesis
Discriminant Analysis
Tissue Distribution
Caspases
Principal Component Analysis
Tumor Burden
Actin Cytoskeleton
Phosphatidylinositol 3-Kinases

ASJC Scopus subject areas

  • Analytical Chemistry
  • Medicine(all)

Cite this

MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model. / Jiang, Lu; Chughtai, Kamila; Purvine, Samuel O.; Bhujwalla, Zaver M; Raman, Venu; Paša-Tolić, Ljiljana; Heeren, Ron M A; Glunde, Kristine.

In: Analytical Chemistry, Vol. 87, No. 12, 16.06.2015, p. 5947-5956.

Research output: Contribution to journalArticle

Jiang, Lu ; Chughtai, Kamila ; Purvine, Samuel O. ; Bhujwalla, Zaver M ; Raman, Venu ; Paša-Tolić, Ljiljana ; Heeren, Ron M A ; Glunde, Kristine. / MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model. In: Analytical Chemistry. 2015 ; Vol. 87, No. 12. pp. 5947-5956.
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AU - Raman, Venu

AU - Paša-Tolić, Ljiljana

AU - Heeren, Ron M A

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