MALDI-ion mobility mass spectrometry of lipids in negative ion mode

Shelley N. Jackson, Damon Barbacci, Thomas Egan, Ernest K. Lewis, J. Albert Schultz, Amina S. Woods

Research output: Contribution to journalArticle

Abstract

Profiling and imaging using MALDI mass spectrometry (MS) allows detection and localization of biomolecules in tissue, of which lipids are a major component. However, due to the in situ nature of this technique, complexity of tissue and need for a chemical matrix, the recorded signal is complex and can be difficult to assign. Ion mobility adds a dimension that provides coarse shape information, separating isobaric lipids, peptides, and oligonucleotides along distinct familial trend lines before mass analysis. Previous work using MALDI-ion mobility mass spectrometry to analyze and image lipids has been conducted mainly in positive ion mode, although several lipid classes ionize preferentially in negative ion mode. This work highlights recent data acquired in negative ion mode to detect glycerophosphoethanolamines (PEs), glycerophosphoserines (PSs), glycerophosphoglycerols (PGs), glycerolphosphoinositols (PIs), glycerophosphates (PAs), sulfatides (STs), and gangliosides from standard tissue extracts and directly from mouse brain tissue. In particular, this study focused on changes in ion mobility based upon lipid head groups, composition of radyl chain (# of carbons and double bonds), diacyl versus plasmalogen species, and hydroxylation of species. Finally, a MALDI-ion mobility imaging run was conducted in negative ion mode, resulting in the successful ion mapping of several lipid species.

Original languageEnglish (US)
Pages (from-to)5001-5007
Number of pages7
JournalAnalytical Methods
Volume6
Issue number14
DOIs
StatePublished - Jul 21 2014
Externally publishedYes

Fingerprint

Lipids
Mass spectrometry
Negative ions
Ions
Tissue
Plasmalogens
Sulfoglycosphingolipids
Glycerophosphates
Imaging techniques
Hydroxylation
Tissue Extracts
Gangliosides
Oligonucleotides
Biomolecules
Peptides
Brain
Carbon
Positive ions
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

Jackson, S. N., Barbacci, D., Egan, T., Lewis, E. K., Albert Schultz, J., & Woods, A. S. (2014). MALDI-ion mobility mass spectrometry of lipids in negative ion mode. Analytical Methods, 6(14), 5001-5007. https://doi.org/10.1039/c4ay00320a

MALDI-ion mobility mass spectrometry of lipids in negative ion mode. / Jackson, Shelley N.; Barbacci, Damon; Egan, Thomas; Lewis, Ernest K.; Albert Schultz, J.; Woods, Amina S.

In: Analytical Methods, Vol. 6, No. 14, 21.07.2014, p. 5001-5007.

Research output: Contribution to journalArticle

Jackson, SN, Barbacci, D, Egan, T, Lewis, EK, Albert Schultz, J & Woods, AS 2014, 'MALDI-ion mobility mass spectrometry of lipids in negative ion mode', Analytical Methods, vol. 6, no. 14, pp. 5001-5007. https://doi.org/10.1039/c4ay00320a
Jackson SN, Barbacci D, Egan T, Lewis EK, Albert Schultz J, Woods AS. MALDI-ion mobility mass spectrometry of lipids in negative ion mode. Analytical Methods. 2014 Jul 21;6(14):5001-5007. https://doi.org/10.1039/c4ay00320a
Jackson, Shelley N. ; Barbacci, Damon ; Egan, Thomas ; Lewis, Ernest K. ; Albert Schultz, J. ; Woods, Amina S. / MALDI-ion mobility mass spectrometry of lipids in negative ion mode. In: Analytical Methods. 2014 ; Vol. 6, No. 14. pp. 5001-5007.
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