Malaria circumsporozoite protein binds to heparan sulfate proteoglycans associated with the surface membrane of hepatocytes

Ute Frevert, Photini Sinnis, Carla Cerami, Wayne Shreffler, Bela Takacs, Victor Nussenzweig

Research output: Contribution to journalArticlepeer-review

Abstract

During feeding by infected mosquitoes, malaria sporozoites are injected into the host’s bloodstream and enterhepatocytes within minutes. The remarkable target cell specificity of this parasite may be explained by the presence of receptors for the region II-plus of the circumsporozoite protein (CS) on the basolateral domain of the plasmamembrane of hepatocytes. We have now identified these receptors as heparan sulfate proteoglycans (HSPG). The binding of CS to the receptors is abolished by heparitinase treatment, indicating that the recognition of region II-plus is viathe glycosaminoglycan chains. We have purified and partially characterized the CS-binding HSPGs from HepG2 cells. Theyhavea molecular weight of 400,000-700,000, are tightly associated with the plasma membrane, and are released from the cellsurface by very mild trypsinization, a property which the CS receptors share with the syndecan family of proteoglycans.

Original languageEnglish (US)
Pages (from-to)1287-1298
Number of pages12
JournalJournal of Experimental Medicine
Volume177
Issue number5
DOIs
StatePublished - May 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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