Major histocompatibility complex independent T cell receptor-antigen interaction: Functional analysis using fluorescein derivatives

Don J. Diamond, Peter Szalay, David Symer, Phyllis Hao, Hyun S. Shin, Renee Z. Dintzis, Howard M. Dintzis, Ellis L. Reinherz, Robert F. Siliciano

Research output: Contribution to journalArticle

Abstract

We have isolated T cell receptor (TCR) cDNAs from fluorescein (FL)-specific human T cell clones (αFLβFL.), and transferred them to TCR β- Jurkat cells in order to study direct FL-binding to the TCR. Using either FLconjugated polymers (FL-polymer) or FL-substituted Sepharose beads, we are able to demonstrate the direct binding of antigen to the T cell surface, and the functional activation of the T cell transfectants. We present evidence against the involvement of major histocompatibility complex (MHC) molecules or antigen presentation in the interaction of FL with the αFLβFL transfectants. Additionally, we have examined the effect of ring substitutions on the FL molecule as well as specific alterations of substituents attached to the 5' position, and we have found that all of them interfere with the functional recognition of the αFLβFL TCR. These experiments demonstrate that TCRs like antibodies have intrinsic affinities for antigen, even without the involvement of MHC molecules.

Original languageEnglish (US)
Pages (from-to)229-241
Number of pages13
JournalJournal of Experimental Medicine
Volume174
Issue number1
DOIs
StatePublished - Jul 1 1991

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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