Maintenance of neurons isolated in bulk from rat brain: incorporation of radiolabeled subtrates

S. E. Poduslo, G. M. McKhann

Research output: Contribution to journalArticle

Abstract

Neurons isolated in bulk from 10- to 15-day-old rat brain can be maintained for 18-24 h. Phase microscopy shows that the cells remain morphologically intact during this time. As a criterion of viability, the incorporation of radiolabeled amino acids and uridine into trichloroacetic acid-insoluble material was selected. The cells are capable of the incorporation both after isolation and after maintenance. The uptake is constant with time, is proportional to substrate concentration, and is inhibited by puromycin and cycloheximide, but not by chloramphenicol. Thus the cells remain viable during this maintenance period. This system may provide a model for the study of the synthesis of specific neuronal components.

Original languageEnglish (US)
Pages (from-to)107-120
Number of pages14
JournalBrain Research
Volume132
Issue number1
DOIs
StatePublished - Aug 19 1977

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Maintenance
Neurons
Brain
Puromycin
Trichloroacetic Acid
Uridine
Chloramphenicol
Cycloheximide
Microscopy
Amino Acids

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Maintenance of neurons isolated in bulk from rat brain : incorporation of radiolabeled subtrates. / Poduslo, S. E.; McKhann, G. M.

In: Brain Research, Vol. 132, No. 1, 19.08.1977, p. 107-120.

Research output: Contribution to journalArticle

Poduslo, S. E. ; McKhann, G. M. / Maintenance of neurons isolated in bulk from rat brain : incorporation of radiolabeled subtrates. In: Brain Research. 1977 ; Vol. 132, No. 1. pp. 107-120.
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