Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae

M. L. Clements, M. M. Levine, C. R. Young, R. E. Black, Robert E Black, R. M. Robins-Browne, J. P. Craig

Research output: Contribution to journalArticle

Abstract

Sera from 147 volunteers were examined for vibriocidal antibody by the tube-dilution method before and after they ingested 103-106 Vibrio cholerae organisms. Titers increased significantly after challenge in 97% of 110 persons who excreted V. cholerae. In 12% of the bacteriologically confirmed infections, the titer increased significantly only to the homologous (Inaba or Ogawa) serotype. Levels of vibriocidal antibody decreased substantially between one and six months after challenge, but they usually remained elevated over base-line values. Levels of antitoxin to V. cholerae were measured by IgG enzyme-linked immunosorbent assay (ELISA), rabbit skin permeability tests, and adrenal cell techniques in 73 pairs of prechallenge and postchallenge sera. Results of the vibriocidal assay agreed most closely with those of the ELISA, the most sensitive antitoxin assay, in serologically detecting clinical and subclinical infections. Recent infection could be accurately serodiagnosed by levels of vibriocidal antibody and antitoxin (by IgG ELISA) in a convalescent-phase serum.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalJournal of Infectious Diseases
Volume145
Issue number4
StatePublished - 1982
Externally publishedYes

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Antitoxins
Vibrio cholerae
Antibody Formation
Eating
Enzyme-Linked Immunosorbent Assay
Antibodies
Immunoglobulin G
Serum
Asymptomatic Infections
Infection
Skin Tests
Volunteers
Permeability
Rabbits

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Clements, M. L., Levine, M. M., Young, C. R., Black, R. E., Black, R. E., Robins-Browne, R. M., & Craig, J. P. (1982). Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae. Journal of Infectious Diseases, 145(4), 465-473.

Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae. / Clements, M. L.; Levine, M. M.; Young, C. R.; Black, R. E.; Black, Robert E; Robins-Browne, R. M.; Craig, J. P.

In: Journal of Infectious Diseases, Vol. 145, No. 4, 1982, p. 465-473.

Research output: Contribution to journalArticle

Clements, ML, Levine, MM, Young, CR, Black, RE, Black, RE, Robins-Browne, RM & Craig, JP 1982, 'Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae', Journal of Infectious Diseases, vol. 145, no. 4, pp. 465-473.
Clements, M. L. ; Levine, M. M. ; Young, C. R. ; Black, R. E. ; Black, Robert E ; Robins-Browne, R. M. ; Craig, J. P. / Magnitude, kinetics, and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae. In: Journal of Infectious Diseases. 1982 ; Vol. 145, No. 4. pp. 465-473.
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