Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent

Piotr Walczak, Jesus Ruiz-Cabello, Dorota Kedziorek, Assaf A. Gilad, Sopo Lin, Bradley Barnett, Lu Qin, Hyam Levitsky, Jeff W Bulte

Research output: Contribution to journalArticle

Abstract

Cellular magnetic resonance imaging (MRI) relies on the use of intracellular contrast agents, primarily iron oxide compounds. Several techniques have been used to efficiently shuttle iron oxides into nonphagocytic cells, but all methods used until now require a prolonged incubation of cells. We hypothesized that instant magnetic labeling of cells could be achieved using electroporation. Neural stem cells (NSCs) and leukocytes from spleen and lymph nodes were suspended in a ferumoxide labeling solution, loaded into cuvettes, and subjected to electromechanical permeabilization using electroporation. Magnetically labeled cells were assayed for labeling efficiency, as well as for potential toxicity or altered function. To confirm the method's applicability to detect cells, MRI experiments were performed at 11.7 T. Magnetoelectroporation of NSCs, as demonstrated by Prussian blue staining, anti-dextran immunostaining, and a quantitative iron uptake assay, proved to be an efficient intracellular magnetic labeling method. Leukocytes including lymphocytes, which are notoriously difficult to label because of their membrane properties and small cytoplasmic volume, also demonstrated a pronounced uptake of ferumoxide. MRI experiments showed that labeled NSCs could be visualized as single cells and cell clusters in gelatin phantoms, and as proliferating cell masses in mouse brain. We have developed a convenient technique for instant magnetic labeling of cells. Because magnetoelectroporation allows the use of ferumoxides approved by the US Food and Drug Administration without additional agents, it has excellent potential for clinical translation.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume2
Issue number2
DOIs
StatePublished - Jun 2006

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Keywords

  • Cell tracking
  • Electroporation
  • Magnetic labeling
  • Magnetic resonance imaging
  • Superparamagnetic iron oxide

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Biotechnology
  • Biophysics
  • Medicine (miscellaneous)

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