Magnetic resonance spectroscopy of siRNA-based cancer therapy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Small interfering RNA (siRNA) is routinely used as a biological tool to silence specific genes, and is under active investigation in cancer treatment strategies. Noninvasive magnetic resonance spectroscopy (MRS) provides the ability to assess the functional effects of siRNA-mediated gene silencing in cultured cancer cells, and following nanoparticle-based delivery in tumors in vivo. Here we describe the use of siRNA to downregulate choline kinase, a critical enzyme in choline phospholipid metabolism of cancer cells and tumors, and the use of 1H MRS of cells and 1H magnetic resonance spectroscopic imaging (MRSI) of tumors to assess the efficacy of the downregulation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages37-47
Number of pages11
DOIs
StatePublished - Jan 1 2015

Publication series

NameMethods in Molecular Biology
Volume1372
ISSN (Print)1064-3745

Keywords

  • Choline metabolism
  • Dual-phase extraction
  • Magnetic resonance spectroscopic imaging (MRSI)
  • Magnetic resonance spectroscopy (MRS)
  • Nanoplex
  • Small interfering RNA (siRNA)
  • Transient transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Penet, M. F., Chen, Z., Mori, N., Krishnamachary, B., & Bhujwalla, Z. M. (2015). Magnetic resonance spectroscopy of siRNA-based cancer therapy. In Methods in Molecular Biology (pp. 37-47). (Methods in Molecular Biology; Vol. 1372). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3148-4_3