Magnetic resonance imaging of ferumoxide-labeled mesenchymal stem cells seeded on collagen scaffolds - Relevance to tissue engineering

John V. Terrovitis, Jeff W Bulte, Sajiram Sarvananthan, Lindsey A. Crowe, Padmini Sarathchandra, Puspa Batten, Eleftherios Sachlos, Adrian H. Chester, Jan T. Czernuszka, David N. Firmin, Patricia M. Taylor, Magdi H. Yacoub

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCs) are a promising candidate cell for tissue engineering. Magnetic resonance imaging (MRI) has been proven effective in visualizing iron-labeled stem cells; however, the efficiency of this approach for visualization of cells seeded on scaffolds intended for use as tissue-engineered heart valves has not been assessed. MSCs were labeled by incubating for 48 h with ferumoxide and poly-L-Iysine as transfecting agent. Any detrimental effect of iron labeling on cell viability, proliferation, and differentiation was examined using appropriate functional assays. Change in the nuclear magnetic relaxation properties of labeled cells was determined using in vitro relaxometry of cells seeded in 3-dimensional collagen gels. Images of labeled and non-labeled cells seeded onto 1% type I bovine collagen scaffolds were obtained using MRI. The presence of intracellular iron in labeled cells was demonstrated using Prussian blue staining, confocal microscopy, and electron microscopy. Cell viability, proliferation, and differentiation were comparable in labeled and non-labeled cells. The T2 relaxation time was 40% to 50% shorter in ferumoxide-labeled cells. Labeled cells seeded on scaffolds appeared as areas of reduced signal intensity in T2 weighted images. Ferumoxide labeling persisted and remained effective even on scans performed 4 weeks after the labeling procedure. Ferumoxide labeling of human MSCs seeded on collagen scaffolds is an effective, non-toxic technique for visualization of these cells using MRI. This technique appears promising for cell tracking in future tissue-engineering applications.

Original languageEnglish (US)
Pages (from-to)2765-2775
Number of pages11
JournalTissue Engineering
Volume12
Issue number10
DOIs
StatePublished - Oct 2006

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Tissue Engineering
Scaffolds (biology)
Stem cells
Mesenchymal Stromal Cells
Tissue engineering
Collagen
Magnetic resonance imaging
Labeling
Magnetic Resonance Imaging
Cell proliferation
Iron
Scaffolds
Visualization
Magnetic relaxation
Confocal microscopy
Relaxation time
Electron microscopy
Assays
Gels
Cell Differentiation

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Magnetic resonance imaging of ferumoxide-labeled mesenchymal stem cells seeded on collagen scaffolds - Relevance to tissue engineering. / Terrovitis, John V.; Bulte, Jeff W; Sarvananthan, Sajiram; Crowe, Lindsey A.; Sarathchandra, Padmini; Batten, Puspa; Sachlos, Eleftherios; Chester, Adrian H.; Czernuszka, Jan T.; Firmin, David N.; Taylor, Patricia M.; Yacoub, Magdi H.

In: Tissue Engineering, Vol. 12, No. 10, 10.2006, p. 2765-2775.

Research output: Contribution to journalArticle

Terrovitis, JV, Bulte, JW, Sarvananthan, S, Crowe, LA, Sarathchandra, P, Batten, P, Sachlos, E, Chester, AH, Czernuszka, JT, Firmin, DN, Taylor, PM & Yacoub, MH 2006, 'Magnetic resonance imaging of ferumoxide-labeled mesenchymal stem cells seeded on collagen scaffolds - Relevance to tissue engineering', Tissue Engineering, vol. 12, no. 10, pp. 2765-2775. https://doi.org/10.1089/ten.2006.12.2765
Terrovitis, John V. ; Bulte, Jeff W ; Sarvananthan, Sajiram ; Crowe, Lindsey A. ; Sarathchandra, Padmini ; Batten, Puspa ; Sachlos, Eleftherios ; Chester, Adrian H. ; Czernuszka, Jan T. ; Firmin, David N. ; Taylor, Patricia M. ; Yacoub, Magdi H. / Magnetic resonance imaging of ferumoxide-labeled mesenchymal stem cells seeded on collagen scaffolds - Relevance to tissue engineering. In: Tissue Engineering. 2006 ; Vol. 12, No. 10. pp. 2765-2775.
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