Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals

R. L. Hackett, P. N. Shevock, S. R. Khan

Research output: Contribution to journalArticle

Abstract

The reaction of Madin-Darby canine kidney cells (MDCK) to potassium oxalate (KOx), calcium oxalate monohydrate (COM) crystals, or a combination of the two was studied. The most noticeable effect of exposure of the cells to either KOx or COM crystals was loss of cells from the monolayer ranging from 20% to 30%, depending upon the particular treatment. Cellular enzyme values in the media were elevated significantly by 12h of exposure, although in specific instances, elevated levels occurred at earlier time periods. As regards the monolayer, trypan blue exclusion was decreased significantly, although amounting to only a 4-5% reduction. Specific tritiated release occurred at 4 and 12 h after exposure to KOx and at 12 h after exposure to crystals. Structurally, COM-cell interactions were complex and extensive endocytosis was noted. Cells were released from culture either as cellcrystal complexes or from the intercellular spaces after exocytosis. When treatment were combined the effects were only slightly additive, but the two treatments potentiated each other: all media enzyme levels (with one exception) were elevated at 2 h, tritiated adenine release was present at 4 h, and there was more extensive cell loss from the culture monolayer. These data suggest that both KOx and COM crystals damage MDCK cells when applied alone, and in concert they act synergistically.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalUrological Research
Volume22
Issue number4
DOIs
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

Calcium Oxalate
Madin Darby Canine Kidney Cells
Oxalates
Oxalic Acid
Trypan Blue
Exocytosis
Extracellular Space
Adenine
Enzymes
Endocytosis
Cell Communication
Cell Culture Techniques

Keywords

  • Adenine
  • Calcium oxalate
  • Electron microscopy
  • MDCK
  • Oxalate
  • Scanning
  • Trypan blue

ASJC Scopus subject areas

  • Urology

Cite this

Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals. / Hackett, R. L.; Shevock, P. N.; Khan, S. R.

In: Urological Research, Vol. 22, No. 4, 07.1994, p. 197-203.

Research output: Contribution to journalArticle

Hackett, R. L. ; Shevock, P. N. ; Khan, S. R. / Madin-Darby canine kidney cells are injured by exposure to oxalate and to calcium oxalate crystals. In: Urological Research. 1994 ; Vol. 22, No. 4. pp. 197-203.
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