Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain

Lynne C Jones, Michelle Tucci, Carmeita Frondoza

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A primary factor limiting the long-term outcome of total joint arthroplasty is the aseptic loosening of prosthetic components. The bone-host interface of an unstable implant is associated with large quantities of wear debris as well as an altered mechanical environment. In vitro cellular studies on the effects of particulate biomaterials have been conducted under static conditions. The principal objective of this study was to test the hypothesis that the application of cyclic tensile strain to cultured macrophages and fibroblasts alters the response of these cells to particulate polymethylmethacrylate. P388D1 macrophages and three human synovial fibroblast cells were exposed to particulate PMMA (0, 0.4, 4.0, and 40.0 mg/well) (n=3) and tested under static or dynamic [cyclic tensile strain (0.5 Hz, 0.2 strain)] conditions. Cellular proliferation was determined by autoradiography following 3H-thymidine incorporation. IL- 1β, TNF-α, and IL-6 were identified using immunocytochemistry. Exposure of macrophages to particulate PMMA and/or cyclic tensile strain resulted in a decrease in DNA synthesis. Neither treatment altered the proliferative capacity of fibroblasts. Macrophages and fibroblasts stained positively for IL-lβ, TNF-α, and IL-6 under all of the experimental conditions tested. Our study provides evidence that macrophages, but not fibroblasts, exhibit decreased proliferative capacity when exposed to particulate PMMA and mechanical stimuli.

Original languageEnglish (US)
Title of host publicationTechnical Papers of ISA
Pages223-230
Number of pages8
Volume464
StatePublished - 2006
Event43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium - Terre Haute, IN, United States
Duration: Apr 7 2006Apr 9 2006

Other

Other43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium
CountryUnited States
CityTerre Haute, IN
Period4/7/064/9/06

Fingerprint

Macrophages
Fibroblasts
Tensile strain
Arthroplasty
Prosthetics
Debris
Biomaterials
Bone
DNA
Cells
Wear of materials

Keywords

  • Cellular response
  • Cyclic tensile strain
  • Fibroblasts
  • Macrophages
  • Particulate PMMA

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

Jones, L. C., Tucci, M., & Frondoza, C. (2006). Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain. In Technical Papers of ISA (Vol. 464, pp. 223-230)

Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain. / Jones, Lynne C; Tucci, Michelle; Frondoza, Carmeita.

Technical Papers of ISA. Vol. 464 2006. p. 223-230.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jones, LC, Tucci, M & Frondoza, C 2006, Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain. in Technical Papers of ISA. vol. 464, pp. 223-230, 43rd Annual Rocky Mountain Bioengineering Symposium and 43rd International ISA Biomedical Sciences Instrumentation Symposium, Terre Haute, IN, United States, 4/7/06.
Jones LC, Tucci M, Frondoza C. Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain. In Technical Papers of ISA. Vol. 464. 2006. p. 223-230
Jones, Lynne C ; Tucci, Michelle ; Frondoza, Carmeita. / Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain. Technical Papers of ISA. Vol. 464 2006. pp. 223-230
@inproceedings{fc6ad54ad3b742f281af48728e44caed,
title = "Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain",
abstract = "A primary factor limiting the long-term outcome of total joint arthroplasty is the aseptic loosening of prosthetic components. The bone-host interface of an unstable implant is associated with large quantities of wear debris as well as an altered mechanical environment. In vitro cellular studies on the effects of particulate biomaterials have been conducted under static conditions. The principal objective of this study was to test the hypothesis that the application of cyclic tensile strain to cultured macrophages and fibroblasts alters the response of these cells to particulate polymethylmethacrylate. P388D1 macrophages and three human synovial fibroblast cells were exposed to particulate PMMA (0, 0.4, 4.0, and 40.0 mg/well) (n=3) and tested under static or dynamic [cyclic tensile strain (0.5 Hz, 0.2 strain)] conditions. Cellular proliferation was determined by autoradiography following 3H-thymidine incorporation. IL- 1β, TNF-α, and IL-6 were identified using immunocytochemistry. Exposure of macrophages to particulate PMMA and/or cyclic tensile strain resulted in a decrease in DNA synthesis. Neither treatment altered the proliferative capacity of fibroblasts. Macrophages and fibroblasts stained positively for IL-lβ, TNF-α, and IL-6 under all of the experimental conditions tested. Our study provides evidence that macrophages, but not fibroblasts, exhibit decreased proliferative capacity when exposed to particulate PMMA and mechanical stimuli.",
keywords = "Cellular response, Cyclic tensile strain, Fibroblasts, Macrophages, Particulate PMMA",
author = "Jones, {Lynne C} and Michelle Tucci and Carmeita Frondoza",
year = "2006",
language = "English (US)",
volume = "464",
pages = "223--230",
booktitle = "Technical Papers of ISA",

}

TY - GEN

T1 - Macrophages and fibroblasts respond differently to PMMA particles and mechanical strain

AU - Jones, Lynne C

AU - Tucci, Michelle

AU - Frondoza, Carmeita

PY - 2006

Y1 - 2006

N2 - A primary factor limiting the long-term outcome of total joint arthroplasty is the aseptic loosening of prosthetic components. The bone-host interface of an unstable implant is associated with large quantities of wear debris as well as an altered mechanical environment. In vitro cellular studies on the effects of particulate biomaterials have been conducted under static conditions. The principal objective of this study was to test the hypothesis that the application of cyclic tensile strain to cultured macrophages and fibroblasts alters the response of these cells to particulate polymethylmethacrylate. P388D1 macrophages and three human synovial fibroblast cells were exposed to particulate PMMA (0, 0.4, 4.0, and 40.0 mg/well) (n=3) and tested under static or dynamic [cyclic tensile strain (0.5 Hz, 0.2 strain)] conditions. Cellular proliferation was determined by autoradiography following 3H-thymidine incorporation. IL- 1β, TNF-α, and IL-6 were identified using immunocytochemistry. Exposure of macrophages to particulate PMMA and/or cyclic tensile strain resulted in a decrease in DNA synthesis. Neither treatment altered the proliferative capacity of fibroblasts. Macrophages and fibroblasts stained positively for IL-lβ, TNF-α, and IL-6 under all of the experimental conditions tested. Our study provides evidence that macrophages, but not fibroblasts, exhibit decreased proliferative capacity when exposed to particulate PMMA and mechanical stimuli.

AB - A primary factor limiting the long-term outcome of total joint arthroplasty is the aseptic loosening of prosthetic components. The bone-host interface of an unstable implant is associated with large quantities of wear debris as well as an altered mechanical environment. In vitro cellular studies on the effects of particulate biomaterials have been conducted under static conditions. The principal objective of this study was to test the hypothesis that the application of cyclic tensile strain to cultured macrophages and fibroblasts alters the response of these cells to particulate polymethylmethacrylate. P388D1 macrophages and three human synovial fibroblast cells were exposed to particulate PMMA (0, 0.4, 4.0, and 40.0 mg/well) (n=3) and tested under static or dynamic [cyclic tensile strain (0.5 Hz, 0.2 strain)] conditions. Cellular proliferation was determined by autoradiography following 3H-thymidine incorporation. IL- 1β, TNF-α, and IL-6 were identified using immunocytochemistry. Exposure of macrophages to particulate PMMA and/or cyclic tensile strain resulted in a decrease in DNA synthesis. Neither treatment altered the proliferative capacity of fibroblasts. Macrophages and fibroblasts stained positively for IL-lβ, TNF-α, and IL-6 under all of the experimental conditions tested. Our study provides evidence that macrophages, but not fibroblasts, exhibit decreased proliferative capacity when exposed to particulate PMMA and mechanical stimuli.

KW - Cellular response

KW - Cyclic tensile strain

KW - Fibroblasts

KW - Macrophages

KW - Particulate PMMA

UR - http://www.scopus.com/inward/record.url?scp=70449112391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70449112391&partnerID=8YFLogxK

M3 - Conference contribution

C2 - 16817612

AN - SCOPUS:70449112391

VL - 464

SP - 223

EP - 230

BT - Technical Papers of ISA

ER -