Macrophage colony-stimulating factor enhances phagocytosis and oxidative burst of mononuclear phagocytes against Penicillium marneffei conidia

Emmanuel Roilides, Caron A. Lyman, Tin Sein, Ruta Petraitiene, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

The responses of rabbit pulmonary alveolar macrophages (PAMs) and elutriated human monocytes (EHMs) to Penicillium marneffei, an emerging dimorphic fungus that may cause fatal disease in human immunodeficiency virus-infected patients, were studied. PAMs and EHMs comparably phagocytosed conidia of two P. marneffei strains in the presence of serum. Electron microscopy showed intraphagosomal destruction of conidia after 12 h. Serum-opsonized conidia elicited significantly more superoxide anion (O2-) release from EHMs compared to non-opsonized conidia, but equivalent O2- amounts to that elicited by serum-opsonized Aspergillus fumigatus conidia. Macrophage colony-stimulating factor (M-CSF) significantly enhanced phagocytosis of P. marneffei conidia by PAMs and EHMs, as shown by light microscopy. Moreover, M-CSF enhanced O2- production by EHMs in response to both serum-opsonized (P

Original languageEnglish (US)
Pages (from-to)19-26
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume36
Issue number1-2
DOIs
StatePublished - May 15 2003
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Fungal Spores
Respiratory Burst
Penicillium
Alveolar Macrophages
Phagocytes
Phagocytosis
Monocytes
Serum
Aspergillus fumigatus
Superoxides
Microscopy
Electron Microscopy
Fungi
HIV
Rabbits
Light

Keywords

  • Macrophage colony-stimulating factor
  • Mononuclear phagocytes
  • Penicillium marneffei

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Macrophage colony-stimulating factor enhances phagocytosis and oxidative burst of mononuclear phagocytes against Penicillium marneffei conidia. / Roilides, Emmanuel; Lyman, Caron A.; Sein, Tin; Petraitiene, Ruta; Walsh, Thomas J.

In: FEMS Immunology and Medical Microbiology, Vol. 36, No. 1-2, 15.05.2003, p. 19-26.

Research output: Contribution to journalArticle

Roilides, Emmanuel ; Lyman, Caron A. ; Sein, Tin ; Petraitiene, Ruta ; Walsh, Thomas J. / Macrophage colony-stimulating factor enhances phagocytosis and oxidative burst of mononuclear phagocytes against Penicillium marneffei conidia. In: FEMS Immunology and Medical Microbiology. 2003 ; Vol. 36, No. 1-2. pp. 19-26.
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