MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells

Yuhua Fu, Pin Lv, Guoquan Yan, Hui Fan, Lu Cheng, Feng Zhang, Yongjun Dang, Hao Wu, Bo Wen

Research output: Contribution to journalArticlepeer-review

Abstract

In the interphase nucleus, chromatin is organized into three-dimensional conformation to coordinate genome functions. The lamina-chromatin association is important to facilitate higher-order chromatin in mammalian cells, but its biological significances and molecular mechanisms remain poorly understood. One obstacle is that the list of lamina-associated proteins remains limited, presumably due to the inherent insolubility of lamina proteins. In this report, we identified 182 proteins associated with lamin B1 (a constitutive component of lamina) in mouse hepatocytes, by adopting virus-based proximity-dependent biotin identification. These proteins are functionally related to biological processes such as chromatin organization. As an example, we validated the association between lamin B1 and core histone macroH2A1, a histone associated with repressive chromatin. Furthermore, we mapped Lamina-associated domains (LADs) in mouse liver cells and found that boundaries of LADs are enriched for macroH2A. More interestingly, knocking-down of macroH2A1 resulted in the release of heterochromatin foci marked by histone lysine 9 trimethylation (H3K9me3) and the decondensation of global chromatin structure. However, down-regulation of lamin B1 led to redistribution of macroH2A1. Taken together, our data indicated that macroH2A1 is associated with lamina and is required to maintain chromatin architecture in mouse liver cells.

Original languageEnglish (US)
Article number17186
JournalScientific reports
Volume5
DOIs
StatePublished - Nov 25 2015

ASJC Scopus subject areas

  • General

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