Abstract
A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 556-569 |
| Number of pages | 14 |
| Journal | Experimental Cell Research |
| Volume | 38 |
| Issue number | 3 |
| State | Published - Jun 1965 |
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- Cell Biology
Cite this
Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles. / Gordis, L.; Nitowsky, H. M.
In: Experimental Cell Research, Vol. 38, No. 3, 06.1965, p. 556-569.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lysosomes in human cell cultures. Kinetics of enzyme release from injured particles
AU - Gordis, L.
AU - Nitowsky, H. M.
PY - 1965/6
Y1 - 1965/6
N2 - A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.
AB - A subcellular fraction prepared from a heteroploid human cell line had the characteristics of lysosomes in that it contained sedimentable acid hydrolases which could be solubilized by adding Triton X-100. Appearance of soluble acid phosphatase activity during acid incubation of these lysosomes was characterized by an initial rapid rise with no further increase until the third hour of incubation. In addition, sedimentable activity increased, suggesting an alteration of permeability of lysosomes to substrate. Ultraviolet irradiation accelerated the release of acid phosphatase during a 90 min period but was not characterized by the initial rapid rise of soluble activity observed with acid incubation. The effects of acid incubation and ultraviolet irradiation were temperature dependent, with no significant release of enzyme activity at 4 °C. Lysosomes from cells grown with 1.5 × 10-4 M prednisolone released less acid phosphatase during acid incubation. Those from cells grown in concentrations of steroids as low as 2 × 10-6 M were similarly affected, but only during the first two hours of acid incubation. Cells grown with 4 × 10-4 M prednisolone showed inhibition of net protein synthesis and no enhancement of lysosome stability.
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UR - http://www.scopus.com/inward/citedby.url?scp=50549192699&partnerID=8YFLogxK
M3 - Article
C2 - 14329387
AN - SCOPUS:50549192699
VL - 38
SP - 556
EP - 569
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 3
ER -