Lysis of mammalian and Sf9 cells

Jennifer Kavran, Daniel J. Leahy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages47-52
Number of pages6
Volume536
ISBN (Print)9780124200708
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume536
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Sf9 Cells
Purification
Proteins

Keywords

  • Cell lysate clarification
  • Eukaryotic cells
  • Mammalian cells
  • Resuspend cells in lysis buffer
  • Sf9 cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Kavran, J., & Leahy, D. J. (2014). Lysis of mammalian and Sf9 cells. In Methods in Enzymology (Vol. 536, pp. 47-52). (Methods in Enzymology; Vol. 536). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420070-8.00004-0

Lysis of mammalian and Sf9 cells. / Kavran, Jennifer; Leahy, Daniel J.

Methods in Enzymology. Vol. 536 Academic Press Inc., 2014. p. 47-52 (Methods in Enzymology; Vol. 536).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kavran, J & Leahy, DJ 2014, Lysis of mammalian and Sf9 cells. in Methods in Enzymology. vol. 536, Methods in Enzymology, vol. 536, Academic Press Inc., pp. 47-52. https://doi.org/10.1016/B978-0-12-420070-8.00004-0
Kavran J, Leahy DJ. Lysis of mammalian and Sf9 cells. In Methods in Enzymology. Vol. 536. Academic Press Inc. 2014. p. 47-52. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420070-8.00004-0
Kavran, Jennifer ; Leahy, Daniel J. / Lysis of mammalian and Sf9 cells. Methods in Enzymology. Vol. 536 Academic Press Inc., 2014. pp. 47-52 (Methods in Enzymology).
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