Lysis of mammalian and Sf9 cells

Jennifer M. Kavran, Daniel J. Leahy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part A
PublisherAcademic Press Inc.
Pages47-52
Number of pages6
ISBN (Print)9780124200708
DOIs
StatePublished - Jan 1 2014

Publication series

NameMethods in Enzymology
Volume536
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell lysate clarification
  • Eukaryotic cells
  • Mammalian cells
  • Resuspend cells in lysis buffer
  • Sf9 cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Kavran, J. M., & Leahy, D. J. (2014). Lysis of mammalian and Sf9 cells. In Laboratory Methods in Enzymology: Protein Part A (pp. 47-52). (Methods in Enzymology; Vol. 536). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420070-8.00004-0