Lysis of human solid tumors by autologous cells sensitized in vitro to alloantigens

J. L. Strausser, A. Mazumder, E. A. Grimm, M. T. Lotze, S. A. Rosenberg

Research output: Contribution to journalArticle

Abstract

Human lymphocytes sensitized in vitro to allogeneic single-donor cells as well as to pools of allogeneic donor cells were evaluated for their cytotoxic potential against autologous fresh solid tumor cells, autologous tissue-cultured tumor and fibroblasts, fresh peripheral blood lymphoid cells (PBL), and Con A blasts of PBL. Using 4-hr 51Cr release assays, pool-sensitized effector cells lysed autologous tissue-cultured tumor and fibroblast targets in 4 of 4 experiments. Similarly, in 2 of 2 experiments with single-donor allosensitized effectors, autologous tissue-cultured tumor was lysed. No lysis of fresh PBL was seen. Significant lysis of fresh autologous tumor target cells was seen in 10 of 12 experiments. Fresh tumor cells, PBL, and Con A blasts appeared equally lysable, since they were equally lysed by effector cells specifically sensitized to alloantigens present on these cells. Cold target inhibition studies demonstrated that cultured tumor and fibroblasts but not PBL shared the determinants recognized by pool-sensitized cells.

Original languageEnglish (US)
Pages (from-to)266-271
Number of pages6
JournalJournal of Immunology
Volume127
Issue number1
StatePublished - Sep 4 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Strausser, J. L., Mazumder, A., Grimm, E. A., Lotze, M. T., & Rosenberg, S. A. (1981). Lysis of human solid tumors by autologous cells sensitized in vitro to alloantigens. Journal of Immunology, 127(1), 266-271.