Lysine succinylation and lysine malonylation in histones

Zhongyu Xie, Junbiao Dai, Lunzhi Dai, Minjia Tan, Zhongyi Cheng, Yeming Wu, Jef D. Boeke, Yingming Zhao

Research output: Contribution to journalArticle

Abstract

Histone protein post-translational modifications (PTMs) are significant for gene expression and DNA repair. Here we report the identification and validation of a new type of PTM in histones, lysine succinylation. The identified lysine succinylated histone peptides were verified by MS/MS of synthetic peptides, HPLC co-elution, and isotopic labeling. We identified 13, 7, 10, and 7 histone lysine succinylation sites in HeLa, mouse embryonic fibroblast, Drosophila S2, and Saccharomyces cerevisiae cells, respectively. We demonstrated that this histone PTM is present in all eukaryotic cells we examined. Mutagenesis of succinylation sites followed by functional assays implied that histone lysine succinylation can cause unique functional consequences. We also identified one and two histone lysine malonylation sites in HeLa and S. cerevisiae cells, respectively. Our results therefore increase potential combinatorial diversity of histone PTMs and suggest possible new connections between histone biology and metabolism.

Original languageEnglish (US)
Pages (from-to)100-107
Number of pages8
JournalMolecular and Cellular Proteomics
Volume11
Issue number5
DOIs
StatePublished - May 2012

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Histones
Lysine
Post Translational Protein Processing
Yeast
Saccharomyces cerevisiae
Mutagenesis
Peptides
Eukaryotic Cells
Fibroblasts
Metabolism
Gene expression
DNA Repair
Labeling
Drosophila
Assays
Repair
High Pressure Liquid Chromatography
Gene Expression
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

Xie, Z., Dai, J., Dai, L., Tan, M., Cheng, Z., Wu, Y., ... Zhao, Y. (2012). Lysine succinylation and lysine malonylation in histones. Molecular and Cellular Proteomics, 11(5), 100-107. https://doi.org/10.1074/mcp.M111.015875

Lysine succinylation and lysine malonylation in histones. / Xie, Zhongyu; Dai, Junbiao; Dai, Lunzhi; Tan, Minjia; Cheng, Zhongyi; Wu, Yeming; Boeke, Jef D.; Zhao, Yingming.

In: Molecular and Cellular Proteomics, Vol. 11, No. 5, 05.2012, p. 100-107.

Research output: Contribution to journalArticle

Xie, Z, Dai, J, Dai, L, Tan, M, Cheng, Z, Wu, Y, Boeke, JD & Zhao, Y 2012, 'Lysine succinylation and lysine malonylation in histones', Molecular and Cellular Proteomics, vol. 11, no. 5, pp. 100-107. https://doi.org/10.1074/mcp.M111.015875
Xie, Zhongyu ; Dai, Junbiao ; Dai, Lunzhi ; Tan, Minjia ; Cheng, Zhongyi ; Wu, Yeming ; Boeke, Jef D. ; Zhao, Yingming. / Lysine succinylation and lysine malonylation in histones. In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 5. pp. 100-107.
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