Lymphotoxin-a and TNF have essential but independent roles in the evolution of the granulomatous response in experimental leprosy

Deanna A. Hagge, Bernadette M. Saunders, Gigi Ebenezer, Nashone A. Ray, Vilma T. Marks, Warwick J. Britton, James L. Krahenbuhl, Linda B. Adams

Research output: Contribution to journalArticle

Abstract

Recent studies identified an association between genetic variants in the lymphotoxin-α (LTα) gene and leprosy. To study the influence of LTα on the control of experimental leprosy, both low- and high-dose Mycobacterium leprae foot pad (FP) infections were evaluated in LTa-deficient chimeric (cLTα -/-) and control chimeric (cB6) mice. Cellular responses to low-dose infection in cLTα -/- mice were dramatically different, with reduced accumulation of CD4 + and CD8 + lymphocytes and macrophages and failure to form granulomas. Growth of M. leprae was contained for 6 months, but augmented late in infection. In contrast, tumor necrosis factor knockout and tumor necrosis factor receptor 1 knockout FPs exhibited extensive inflammatory infiltration with an increase in M. leprae growth throughout infection. Following high-dose infection, cB6 FP induration peaked at 4 weeks and was maintained for 12 weeks. Induration was not sustained in cLTα -/- FPs that contained few lymphocytes and no granulomas. There was a reduction in the expression levels of inflammatory cytokines, chemokines, and chemokine receptors, including nitric oxide synthase 2, vascular cell adhesion molecule, and intercellular cell adhesion molecule. Furthermore, cLTα -/- popliteal lymph nodes contained a higher proportion of naive CD44 loCD62L hi T cells than cB6 mice, suggestive of reduced T cell activation. Therefore, both LTa and tumor necrosis factor are essential for the regulation of the granuloma, but they have distinctive roles in the recruitment of lymphocytes and maintenance of the granulomatous response during chronic M. leprae infection.

Original languageEnglish (US)
Pages (from-to)1379-1389
Number of pages11
JournalAmerican Journal of Pathology
Volume174
Issue number4
DOIs
StatePublished - Apr 2009

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Lymphotoxin-alpha
Leprosy
Mycobacterium leprae
Granuloma
Infection
Cell Adhesion Molecules
Lymphocytes
Foot
Tumor Necrosis Factor-alpha
T-Lymphocytes
Mycobacterium Infections
Vascular Cell Adhesion Molecule-1
Tumor Necrosis Factor Receptors
Chemokine Receptors
Growth
Chemokines
Nitric Oxide Synthase
Lymph Nodes
Macrophages
Maintenance

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

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Lymphotoxin-a and TNF have essential but independent roles in the evolution of the granulomatous response in experimental leprosy. / Hagge, Deanna A.; Saunders, Bernadette M.; Ebenezer, Gigi; Ray, Nashone A.; Marks, Vilma T.; Britton, Warwick J.; Krahenbuhl, James L.; Adams, Linda B.

In: American Journal of Pathology, Vol. 174, No. 4, 04.2009, p. 1379-1389.

Research output: Contribution to journalArticle

Hagge, Deanna A. ; Saunders, Bernadette M. ; Ebenezer, Gigi ; Ray, Nashone A. ; Marks, Vilma T. ; Britton, Warwick J. ; Krahenbuhl, James L. ; Adams, Linda B. / Lymphotoxin-a and TNF have essential but independent roles in the evolution of the granulomatous response in experimental leprosy. In: American Journal of Pathology. 2009 ; Vol. 174, No. 4. pp. 1379-1389.
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