Lymphocytocidal lymphocyte trapping: Dynamic characteristics of the phenomenon and the nonmacrophage identity of the lymphocytocidal trapping cell

J. B. Margolick, R. P. Sherwin

Research output: Contribution to journalArticle

Abstract

The dynamic characteristics of lymphocytocidal lymphocyte trapping were investigated by tissue culture studies of 166 human lymphoid organs that were surgically resected. For the dynamic studies, time lapse cinephotomicrographic film records of 61 lymphocyte deaths associated with 21 lymphocytocidal trapping cells (LTC) were analyzed. The results confirmed and extended earlier findings, namely that lymphocytocidal lymphocyte trapping is characterized by a) lymphocyte immobilization, i.e., a sudden restriction in lymphocyte movement to one region of the LTC, b) localization of immobilization to the juxtanuclear region of the LTC, i.e., less than 20 μm from the LTC nucleus, and c) rapid death and degeneration of the immobilized lymphocyte, i.e., lymphocyte death occurred as early as 3.5 min after immobilization, with an average time of 108 min. This sequence of events also occurred synchronously for groups of up to 4 lymphocytes, further supporting the validity of the lymphocytocidal trapping interpretation. Thus, lymphocytocidal trapping is a real and reproducible phenomenon which reflects an as yet undetermined lymphocytocidal property of the LTC. With respect to LTC identity, the LTC differed from macrophages that were present in the same cultures in that they did not phagocytose polystyrene latex beads or anthracotic pigment, failed to internalize exogenous horseradish peroxidase, lacked nonspecific esterase, and had weaker cytochemical reactions for ATPase, acid phosphatase, and other enzymes. Also, this study and earlier observations have shown that the LTC lacks ruffling, pseudopodia and ameboid motion. It is concluded that the LTC is not a macrophage. Data concerning distribution of the LTC in lymphoid tissues of human and other species are presented in an accompanying report.

Original languageEnglish (US)
Pages (from-to)151-165
Number of pages15
JournalRES Journal of the Reticuloendothelial Society
Volume28
Issue number2
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Lymphocytocidal lymphocyte trapping: Dynamic characteristics of the phenomenon and the nonmacrophage identity of the lymphocytocidal trapping cell'. Together they form a unique fingerprint.

  • Cite this