Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc receptors

J. Yodoi, K. Ishizaka

Research output: Contribution to journalArticle

Abstract

Human peripheral blood lymphocytes from ragweed-sensitive individuals were fractionated by a rosette technique with neuraminidase-treated sheep erythrocytes (En). A significant number of Fc(ε)R-bearing cells were detected in the T cell fraction by rosette formation with ox erythrocytes coated with human IgE (E'-hIgE), or by the binding of fluoresceinated F(ab')2 fragments of anti-IgE complexed with IgE. The majority of these lymphocytes in the T cell fraction formed mixed rosettes with En and E'-hIgE. T cells bearing Fc(ε)R were demonstrated in the mesenteric lymph node cells from rats infected with Nippostrongylus brasiliensis (Nb); about 3% of the total cells in a B-depleted fraction formed rosettes with ox erythrocytes coated with rat IgE (E'-rIgE). Most of the rosette-forming cells (RFC) in the fraction were stained by antibodies specific for rat T cells in immunofluorescence. A substantial proportion (5 to 10%) of lymphocytes in rat-activated T cells, which were prepared by transfer of Lewis strain thymocytes to Sprague-Dawley rats, formed rosettes with E'-rIgE, and essentially all of the RFC were stained by anti-T cell antibodies. The results indicated that a subset of human and rat T cells possess Fc(ε)R. Evidence was obtained that Fc(ε)R on T cells are distinct from Fc receptors for IgG (Fc(γ)R), which were demonstrated by rosette formation with ox erythrocytes coated with rabbit IgG antibodies (EA). Mixed rosette assays showed that more than a half of Fc(ε)R-bearing human T cells failed to bind EA. Similarly, the majority of Fc(ε)R-bearing T cells in the lymph nodes of Nb-infected rats failed to bind EA. In activated T cell preparations, however, about three-fourths of Fc(ε)R-bearing cells formed mixed rosettes with EA, indicating that some T cells bear both Fc(ε)R and Fc(γ)R.

Original languageEnglish (US)
Pages (from-to)2577-2583
Number of pages7
JournalJournal of Immunology
Volume122
Issue number6
StatePublished - 1979

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Fc Receptors
Immunoglobulin E
Lymphocytes
T-Lymphocytes
Erythrocytes
Nippostrongylus
Rosette Formation
Antibodies
Immunoglobulin G
Lymph Nodes
Ambrosia
Neuraminidase
Thymocytes
Fluorescent Antibody Technique
Sprague Dawley Rats
Sheep

ASJC Scopus subject areas

  • Immunology

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Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc receptors. / Yodoi, J.; Ishizaka, K.

In: Journal of Immunology, Vol. 122, No. 6, 1979, p. 2577-2583.

Research output: Contribution to journalArticle

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abstract = "Human peripheral blood lymphocytes from ragweed-sensitive individuals were fractionated by a rosette technique with neuraminidase-treated sheep erythrocytes (En). A significant number of Fc(ε)R-bearing cells were detected in the T cell fraction by rosette formation with ox erythrocytes coated with human IgE (E'-hIgE), or by the binding of fluoresceinated F(ab')2 fragments of anti-IgE complexed with IgE. The majority of these lymphocytes in the T cell fraction formed mixed rosettes with En and E'-hIgE. T cells bearing Fc(ε)R were demonstrated in the mesenteric lymph node cells from rats infected with Nippostrongylus brasiliensis (Nb); about 3{\%} of the total cells in a B-depleted fraction formed rosettes with ox erythrocytes coated with rat IgE (E'-rIgE). Most of the rosette-forming cells (RFC) in the fraction were stained by antibodies specific for rat T cells in immunofluorescence. A substantial proportion (5 to 10{\%}) of lymphocytes in rat-activated T cells, which were prepared by transfer of Lewis strain thymocytes to Sprague-Dawley rats, formed rosettes with E'-rIgE, and essentially all of the RFC were stained by anti-T cell antibodies. The results indicated that a subset of human and rat T cells possess Fc(ε)R. Evidence was obtained that Fc(ε)R on T cells are distinct from Fc receptors for IgG (Fc(γ)R), which were demonstrated by rosette formation with ox erythrocytes coated with rabbit IgG antibodies (EA). Mixed rosette assays showed that more than a half of Fc(ε)R-bearing human T cells failed to bind EA. Similarly, the majority of Fc(ε)R-bearing T cells in the lymph nodes of Nb-infected rats failed to bind EA. In activated T cell preparations, however, about three-fourths of Fc(ε)R-bearing cells formed mixed rosettes with EA, indicating that some T cells bear both Fc(ε)R and Fc(γ)R.",
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