TY - JOUR
T1 - Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc receptors
AU - Yodoi, J.
AU - Ishizaka, K.
PY - 1979/12/1
Y1 - 1979/12/1
N2 - Human peripheral blood lymphocytes from ragweed-sensitive individuals were fractionated by a rosette technique with neuraminidase-treated sheep erythrocytes (En). A significant number of Fc(ε)R-bearing cells were detected in the T cell fraction by rosette formation with ox erythrocytes coated with human IgE (E'-hIgE), or by the binding of fluoresceinated F(ab')2 fragments of anti-IgE complexed with IgE. The majority of these lymphocytes in the T cell fraction formed mixed rosettes with En and E'-hIgE. T cells bearing Fc(ε)R were demonstrated in the mesenteric lymph node cells from rats infected with Nippostrongylus brasiliensis (Nb); about 3% of the total cells in a B-depleted fraction formed rosettes with ox erythrocytes coated with rat IgE (E'-rIgE). Most of the rosette-forming cells (RFC) in the fraction were stained by antibodies specific for rat T cells in immunofluorescence. A substantial proportion (5 to 10%) of lymphocytes in rat-activated T cells, which were prepared by transfer of Lewis strain thymocytes to Sprague-Dawley rats, formed rosettes with E'-rIgE, and essentially all of the RFC were stained by anti-T cell antibodies. The results indicated that a subset of human and rat T cells possess Fc(ε)R. Evidence was obtained that Fc(ε)R on T cells are distinct from Fc receptors for IgG (Fc(γ)R), which were demonstrated by rosette formation with ox erythrocytes coated with rabbit IgG antibodies (EA). Mixed rosette assays showed that more than a half of Fc(ε)R-bearing human T cells failed to bind EA. Similarly, the majority of Fc(ε)R-bearing T cells in the lymph nodes of Nb-infected rats failed to bind EA. In activated T cell preparations, however, about three-fourths of Fc(ε)R-bearing cells formed mixed rosettes with EA, indicating that some T cells bear both Fc(ε)R and Fc(γ)R.
AB - Human peripheral blood lymphocytes from ragweed-sensitive individuals were fractionated by a rosette technique with neuraminidase-treated sheep erythrocytes (En). A significant number of Fc(ε)R-bearing cells were detected in the T cell fraction by rosette formation with ox erythrocytes coated with human IgE (E'-hIgE), or by the binding of fluoresceinated F(ab')2 fragments of anti-IgE complexed with IgE. The majority of these lymphocytes in the T cell fraction formed mixed rosettes with En and E'-hIgE. T cells bearing Fc(ε)R were demonstrated in the mesenteric lymph node cells from rats infected with Nippostrongylus brasiliensis (Nb); about 3% of the total cells in a B-depleted fraction formed rosettes with ox erythrocytes coated with rat IgE (E'-rIgE). Most of the rosette-forming cells (RFC) in the fraction were stained by antibodies specific for rat T cells in immunofluorescence. A substantial proportion (5 to 10%) of lymphocytes in rat-activated T cells, which were prepared by transfer of Lewis strain thymocytes to Sprague-Dawley rats, formed rosettes with E'-rIgE, and essentially all of the RFC were stained by anti-T cell antibodies. The results indicated that a subset of human and rat T cells possess Fc(ε)R. Evidence was obtained that Fc(ε)R on T cells are distinct from Fc receptors for IgG (Fc(γ)R), which were demonstrated by rosette formation with ox erythrocytes coated with rabbit IgG antibodies (EA). Mixed rosette assays showed that more than a half of Fc(ε)R-bearing human T cells failed to bind EA. Similarly, the majority of Fc(ε)R-bearing T cells in the lymph nodes of Nb-infected rats failed to bind EA. In activated T cell preparations, however, about three-fourths of Fc(ε)R-bearing cells formed mixed rosettes with EA, indicating that some T cells bear both Fc(ε)R and Fc(γ)R.
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M3 - Article
C2 - 109532
AN - SCOPUS:0018748142
SN - 0022-1767
VL - 122
SP - 2577
EP - 2583
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -