Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies

R. S. Fishel, A. Barbul, W. E. Beschorner, H. L. Wasserkrug, G. Efron

Research output: Contribution to journalArticle

Abstract

To investigate lymphocyte participation in wound healing, the migration of T lymphocyte subsets into healing wounds and subcutaneously implanted polyvinyl alcohol sponges was studied. Frozen sections of 5-, 7-, and 10-day-old incisional wounds and sponges from Lewis rats were stained with mouse anti-rat monoclonal antibodies. Cellular staining to OX1 (all leucocyte), W3/25 (helper/effector T lymphocytes), and OX8 (suppressor/cytotoxic T lymphocytes) was quantitated in two arbitrarily defined areas based on maximal cellular infiltration: (1) the superficial wound, down to and including the papillary dermis, and (2) the deep wound, the reticular dermis. Five-day wounds were significantly more cellular than 10-day wounds in the deep portion (p <0.05) and somewhat more cellular in the superficial section (p <0.10). Approximately 2:1 W3/25 to OX8 ratios were noted for wound strips on all days. At 5 and 10 days there are twice as many W3/25 and OX8 labeled cells in the deep wound as in the superficial portion. At 7 days there is a peak in surface W3/25 and OX8 lymphocytes, whereas the deep population remains constant. Seven- and 10-day sponge granulomas demonstrate ratios similar to the wound strips (5-day sponge lymphocytic infiltraton was insufficient to count). The data demonstrate that lymphocyte subpopulation participation in wound healing is a dynamic and distinctive process.

Original languageEnglish (US)
Pages (from-to)25-29
Number of pages5
JournalAnnals of Surgery
Volume206
Issue number1
StatePublished - 1987
Externally publishedYes

Fingerprint

Wound Healing
Monoclonal Antibodies
Lymphocytes
Wounds and Injuries
Porifera
Dermis
Polyvinyl Alcohol
Lymphocyte Subsets
Frozen Sections
T-Lymphocyte Subsets
Cytotoxic T-Lymphocytes
Helper-Inducer T-Lymphocytes
Granuloma
Leukocytes
Staining and Labeling
Population

ASJC Scopus subject areas

  • Surgery

Cite this

Fishel, R. S., Barbul, A., Beschorner, W. E., Wasserkrug, H. L., & Efron, G. (1987). Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies. Annals of Surgery, 206(1), 25-29.

Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies. / Fishel, R. S.; Barbul, A.; Beschorner, W. E.; Wasserkrug, H. L.; Efron, G.

In: Annals of Surgery, Vol. 206, No. 1, 1987, p. 25-29.

Research output: Contribution to journalArticle

Fishel, RS, Barbul, A, Beschorner, WE, Wasserkrug, HL & Efron, G 1987, 'Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies', Annals of Surgery, vol. 206, no. 1, pp. 25-29.
Fishel RS, Barbul A, Beschorner WE, Wasserkrug HL, Efron G. Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies. Annals of Surgery. 1987;206(1):25-29.
Fishel, R. S. ; Barbul, A. ; Beschorner, W. E. ; Wasserkrug, H. L. ; Efron, G. / Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies. In: Annals of Surgery. 1987 ; Vol. 206, No. 1. pp. 25-29.
@article{bcea06c0953f456faa4975b2b6c3b81a,
title = "Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies",
abstract = "To investigate lymphocyte participation in wound healing, the migration of T lymphocyte subsets into healing wounds and subcutaneously implanted polyvinyl alcohol sponges was studied. Frozen sections of 5-, 7-, and 10-day-old incisional wounds and sponges from Lewis rats were stained with mouse anti-rat monoclonal antibodies. Cellular staining to OX1 (all leucocyte), W3/25 (helper/effector T lymphocytes), and OX8 (suppressor/cytotoxic T lymphocytes) was quantitated in two arbitrarily defined areas based on maximal cellular infiltration: (1) the superficial wound, down to and including the papillary dermis, and (2) the deep wound, the reticular dermis. Five-day wounds were significantly more cellular than 10-day wounds in the deep portion (p <0.05) and somewhat more cellular in the superficial section (p <0.10). Approximately 2:1 W3/25 to OX8 ratios were noted for wound strips on all days. At 5 and 10 days there are twice as many W3/25 and OX8 labeled cells in the deep wound as in the superficial portion. At 7 days there is a peak in surface W3/25 and OX8 lymphocytes, whereas the deep population remains constant. Seven- and 10-day sponge granulomas demonstrate ratios similar to the wound strips (5-day sponge lymphocytic infiltraton was insufficient to count). The data demonstrate that lymphocyte subpopulation participation in wound healing is a dynamic and distinctive process.",
author = "Fishel, {R. S.} and A. Barbul and Beschorner, {W. E.} and Wasserkrug, {H. L.} and G. Efron",
year = "1987",
language = "English (US)",
volume = "206",
pages = "25--29",
journal = "Annals of Surgery",
issn = "0003-4932",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Lymphocyte participation in wound healing. Morphologic assessment using monoclonal antibodies

AU - Fishel, R. S.

AU - Barbul, A.

AU - Beschorner, W. E.

AU - Wasserkrug, H. L.

AU - Efron, G.

PY - 1987

Y1 - 1987

N2 - To investigate lymphocyte participation in wound healing, the migration of T lymphocyte subsets into healing wounds and subcutaneously implanted polyvinyl alcohol sponges was studied. Frozen sections of 5-, 7-, and 10-day-old incisional wounds and sponges from Lewis rats were stained with mouse anti-rat monoclonal antibodies. Cellular staining to OX1 (all leucocyte), W3/25 (helper/effector T lymphocytes), and OX8 (suppressor/cytotoxic T lymphocytes) was quantitated in two arbitrarily defined areas based on maximal cellular infiltration: (1) the superficial wound, down to and including the papillary dermis, and (2) the deep wound, the reticular dermis. Five-day wounds were significantly more cellular than 10-day wounds in the deep portion (p <0.05) and somewhat more cellular in the superficial section (p <0.10). Approximately 2:1 W3/25 to OX8 ratios were noted for wound strips on all days. At 5 and 10 days there are twice as many W3/25 and OX8 labeled cells in the deep wound as in the superficial portion. At 7 days there is a peak in surface W3/25 and OX8 lymphocytes, whereas the deep population remains constant. Seven- and 10-day sponge granulomas demonstrate ratios similar to the wound strips (5-day sponge lymphocytic infiltraton was insufficient to count). The data demonstrate that lymphocyte subpopulation participation in wound healing is a dynamic and distinctive process.

AB - To investigate lymphocyte participation in wound healing, the migration of T lymphocyte subsets into healing wounds and subcutaneously implanted polyvinyl alcohol sponges was studied. Frozen sections of 5-, 7-, and 10-day-old incisional wounds and sponges from Lewis rats were stained with mouse anti-rat monoclonal antibodies. Cellular staining to OX1 (all leucocyte), W3/25 (helper/effector T lymphocytes), and OX8 (suppressor/cytotoxic T lymphocytes) was quantitated in two arbitrarily defined areas based on maximal cellular infiltration: (1) the superficial wound, down to and including the papillary dermis, and (2) the deep wound, the reticular dermis. Five-day wounds were significantly more cellular than 10-day wounds in the deep portion (p <0.05) and somewhat more cellular in the superficial section (p <0.10). Approximately 2:1 W3/25 to OX8 ratios were noted for wound strips on all days. At 5 and 10 days there are twice as many W3/25 and OX8 labeled cells in the deep wound as in the superficial portion. At 7 days there is a peak in surface W3/25 and OX8 lymphocytes, whereas the deep population remains constant. Seven- and 10-day sponge granulomas demonstrate ratios similar to the wound strips (5-day sponge lymphocytic infiltraton was insufficient to count). The data demonstrate that lymphocyte subpopulation participation in wound healing is a dynamic and distinctive process.

UR - http://www.scopus.com/inward/record.url?scp=0023176582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023176582&partnerID=8YFLogxK

M3 - Article

C2 - 2955751

AN - SCOPUS:0023176582

VL - 206

SP - 25

EP - 29

JO - Annals of Surgery

JF - Annals of Surgery

SN - 0003-4932

IS - 1

ER -