Lymphocyte activation induces rapid changes in nuclear and cytoplasmic glycoproteins

Kelly P. Kearse, Gerald Warren Hart

Research output: Contribution to journalArticle

Abstract

A unique form of nucleoplasmic and cytoplasmic protein glycosylation, O-linked GlcNAc, (O-GlcNAc) is present on proteins ranging from those of yeast to man, including many chromatin proteins, transcription factors, nuclear pore proteins, and certain types of cytoskeletal proteins. In this report we have studied the effects of cellular activation on O-GlcNAc-modified proteins, using T lymphocytes as a model system. Results indicate that the apparent levels of O-GlcNAc on many nuclear proteins increases rapidly after lymphocyte activation, returning to control levels after a few hours. In contrast, the apparent levels of O-GlcNAc on a distinct population of cytosolic proteins decreases rapidly after cellular activation and also returns to control levels after a few hours. These data are consistent with the hypothesis that O-GlcNAc is a regulatory modification and suggest that O-GlcNAc modification may play an important role in the early stages of T-lymphocyte activation.

Original languageEnglish (US)
Pages (from-to)1701-1705
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number5
StatePublished - 1991

    Fingerprint

Keywords

  • Nucleoplasmic and cytoplasmic glycosylation
  • O-linked N-acetylglucosamine
  • Pro-Glu-Ser
  • Thr regions

ASJC Scopus subject areas

  • General
  • Genetics

Cite this